ARHGDIA Antibody (OACA00078)

Catalog No: OACA00078

Size:100ug

All lanes : Rho GDP-dissociation inhibitor 1 antibody at2 µg/ml
Lane 1 :EC109 whole cell lysate
Lane 2 :293T whole cell lysate
Secondary Goat polyclonal to Rabbit IgG at 1/15000 dilution
Predicted band size : 22.4 kDa
Observed band size: 40 kDa

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Conjugation: Unconjugated
• Application: Enzyme-linked immunosorbent assay|Immunohistochemistry|Western blot
• Reconstitution and Storage: -20°C or -80°C
• Immunogen: Recombinant Human Rho GDP-dissociation inhibitor 1 protein (2-204AA)
• Purification: Protein G Purified
• Purity: > 95% by Caprylic Acid Ammonium Sulfate Precipitation
• Application Info: ELISA: Use at an assay dependent dilution
  WB: 1:500 ~ 2000
  IHC: 1:20 ~ 200
• Reference: [1] Identification of two human Rho GDP dissociation inhibitor proteins whose overexpression leads to disruption of the actin cytoskeleton.'Leffers H., Nielsen M.S., Andersen A.H., Honore B., Madsen P., Vandekerckhove J., Celis J.E.Exp. Cell Res. 209:165-174(1993)
  [2] Molecular cloning of human rho GDI.'Maeda A., Kaibuchi K., Takai Y.
  [3] cDNA sequence of human rho GDP dissociation inhibitor.'Chuang T.H., Bokoch G.M.
  [4] Genomic sequence of a human rho GDP dissociation inhibitor (GDI).'Mulheron J.G., Schwinn D.A., Caron M.G., Liggett S.B.

Target Info

• Gene Symbol: ARHGDIA
• Gene Full Name: Rho GDP dissociation inhibitor alpha
• Alias Symbols: epididymis secretory sperm binding protein Li 47e;GDIA1;GDP-dissociation inhibitor, aplysia RAS-related 1;HEL-S-47e;NPHS8;Rho GDP dissociation inhibitor (GDI) alpha;rho GDP-dissociation inhibitor 1;RHOGDI;Rho-GDI alpha;RHOGDI-1.
• NCBI Gene Id: 396
• Protein Name: Rho GDP-dissociation inhibitor 1
• Description of Target: Controls Rho proteins homeostasis. Regulates the GDP/GTP exchange reaction of the Rho proteins by inhibiting the dissociation of GDP from them, and the subsequent binding of GTP to them. Retains Rho proteins such as CDC42, RAC1 and RHOA in an inactive cytosolic pool, regulating their stability and protecting them from degradation. Actively involved in the recycling and distribution of activated Rho GTPases in the cell, mediates extraction from membranes of both inactive and activated molecules due its exceptionally high affinity for prenylated forms. Through the modulation of Rho proteins, may play a role in cell motility regulation. In glioma cells, inhibits cell migration and invasion by mediating the signals of SEMA5A and PLXNB3 that lead to inactivation of RAC1.
• Uniprot ID: P52565
• Protein Accession #: NP_001172006.1; NP_001172007.1; NP_001288169.1; NP_001288170.1; NP_001288171.1; NP_001288172.1; NP_004300.1
• Nucleotide Accession #: NM_001185077.2; NM_001185078.2; NM_001301240.1; NM_001301241.1; NM_001301242.1; NM_001301243.1; NM_004309.5