ARHGAP4 Antibody (OAGA00224)

Catalog No: OAGA00224

Size:100 ul

Sample (30 ug of whole cell lysate)
A: JurKat
7.5% SDS PAGE
OAGA00224 diluted at 1:1000

Immunohistochemical analysis of paraffin-embedded Colon ca, using ARHGAP4(OAGA00224) antibody at 1:500 dilution.

Immunofluorescence analysis of paraformaldehyde-fixed A549, using Rho GAP4(OAGA00224) antibody at 1:200 dilution.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: ICC, IF, IHC-FFPE, WB
• Additional Information: Background: This gene encodes a member of the rhoGAP family of proteins which play a role in the regulation of small GTP-binding proteins belonging to the RAS superfamily. The protein encoded by the orthologous gene in rat is localized to the Golgi complex and can redistribute to microtubules. The rat protein stimulates the activity of some Rho GTPases in vitro. Genomic deletions of this gene and a neighboring gene have been found in patients with nephrogenic diabetes insipidus. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
  Positive Controls: Jurkat, NT2D1
  Cellular Localization: Cytoplasm
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Recombinant fragment corresponding to a region within amino acids 1 and 280 of ARHGAP4 (Uniprot ID#P98171)
• Purification: Purified by antigen-affinity chromatography.
• Concentration: 1 mg/ml
• Application Info: Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
• Storage Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Target Info

• Gene Symbol: ARHGAP4
• Gene Full Name: Rho GTPase-activating protein 4
• Alias Symbols: C1, RGC1, p115, SrGAP4, RhoGAP4
• NCBI Gene Id: 393
• Protein Name: Rho GTPase-activating protein 4
• Uniprot ID: P98171
• Protein Accession #: NP_001158213.1
• Molecular Weight: 105 kDa