Arginine vasopressin ELISA Kit (OKEH02585)

Catalog No: OKEH02585

Size:96 Wells

Arginine vasopressin ELISA Kit () (OKEH02585) standard curve.

Product Info

• Predicted Species Reactivity: All
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology Arginine vasopressin ELISA Kit () (OKEH02585) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Arginine vasopressin antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Arginine vasopressin and incubated. The Arginine vasopressin found in the sample or standards competes with the biotinylated Arginine vasopressin for limited binding sites on the immobilized anti-Arginine vasopressin antibody. Excess unbound biotinylated Arginine vasopressin and sample or standard Arginine vasopressin is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Arginine vasopressin captured in the well and inversely proportional to the amount of Arginine vasopressin which was contained in the sample or standard.
• ELISA Kit Range: 31.2 - 2000pg/mL
• ELISA Kit Reproducibility: Mean Intra-assay CV%: < 4.1% (n = 20)
  Mean Inter-assay CV%: < 7.5% (n = 20)
• ELISA Kit Component:
  • Anti-Arginine vasopressin Microplate: 96 Wells (12 x 8 Well strips)
  • Arginine vasopressin Lyophilized Standard: 2 x 2,000 pg
  • Sample Diluent: 1 x 20 mL
  • 100X Arginine vasopressin-Biotin Complex: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Biotin Complex Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Target Abbr: AVPP
  Target Name: Arginine vasopressin
  Pubchem: 644077
  Chemical formula: C46H65N15O12S2
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, plasma, tissue homogenates, cell culture supernatants and other biological fluids
• Sensitivity: 15.1 pg/mL
• Specificity: Natural and recombinant General Arginine vasopressin
• Publications:

  Timeline of Changes in Biomarkers Associated with Spinal Cord Injury-Induced Polyuria. Neurotrauma Rep. 2, 462-475 (2021). 34901942

• Assay Info: Assay Methodology: Quantitative Competitive ELISA

Target Info

• Alias Symbols: ARGIPRESSIN; 113-79-1; Arginine vasopressin; Argipressine; Pitressin; Vasopressin (arginine form)
• Protein Name: Arginine vasopressin
• Description of Target: Vasopressin is the predominant form of mammalian antidiuretic hormone. It is a nonapeptide containing an arginine at residue 8 and two disulfide-linked cysteines at residues of 1 and 6. Arg-vasopressin is used to treat diabetes insipidus or to improve vasomotor tone and blodd pressure.