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Catalog No: OKEH02585
Size:96T
Price: $625.00
SKU
OKEH02585
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
COA DatasheetPrintable COA datasheet for OKEH02585
Product Info
Predicted Species ReactivityAll Species
ApplicationEnzyme-linked Immunosorbent assay-Competitive
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology Arginine vasopressin ELISA Kit () (OKEH02585) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Arginine vasopressin antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Arginine vasopressin and incubated. The Arginine vasopressin found in the sample or standards competes with the biotinylated Arginine vasopressin for limited binding sites on the immobilized anti-Arginine vasopressin antibody. Excess unbound biotinylated Arginine vasopressin and sample or standard Arginine vasopressin is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Arginine vasopressin captured in the well and inversely proportional to the amount of Arginine vasopressin which was contained in the sample or standard.
ELISA Kit Range31.2-2000pg/mL
ELISA Kit ReproducibilityMean Intra-assay CV%: < 4.1% (n = 20)
Mean Inter-assay CV%: < 7.5% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-Arginine vasopressin Microplate96 Wells (12 x 8 Well strips)
Arginine vasopressin Lyophilized Standard2 x 2,000 pg
Sample Diluent1 x 20 mL
100X Arginine vasopressin-Biotin Complex1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Biotin Complex Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Additional InformationTarget Abbr: AVPP
Target Name: Arginine vasopressin
::Pubchem: 644077
::Chemical formula: C46H65N15O12S2
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, plasma, tissue homogenates, cell culture supernatants and other biological fluids
Sensitivity15.1 pg/mL
SpecificityNatural and recombinant General Arginine vasopressin
Publications

Timeline of Changes in Biomarkers Associated with Spinal Cord Injury-Induced Polyuria. Neurotrauma Rep. 2, 462-475 (2021). 34901942

Assay InfoAssay Methodology: Quantitative Competitive ELISA
Description
Alias SymbolsARGIPRESSIN; 113-79-1; Arginine vasopressin; Argipressine; Pitressin; Vasopressin (arginine form)
Protein NameArginine vasopressin
Description of TargetVasopressin is the predominant form of mammalian antidiuretic hormone. It is a nonapeptide containing an arginine at residue 8 and two disulfide-linked cysteines at residues of 1 and 6. Arg-vasopressin is used to treat diabetes insipidus or to improve vasomotor tone and blodd pressure.
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