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Catalog No: OKCA01842
Size:96 Wells
Price: $754.00
SKU
OKCA01842
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityRat
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3.5 Hours
ELISA Kit Linearity
DilutionMean Recovery (%)Range (%)
ELISA Kit PrincipleAviva Systems Biology ARG2 ELISA Kit (Rat) (OKCA01842) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ARG2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ARG2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample ARG2 captured in the well.
ELISA Kit Range0.78 - 50 ng/mL
ELISA Kit Recovery
SampleMean Recovery (%)Range (%)
Serum (n=5)105101-110
EDTA Plasma (n=4)8683-89
ELISA Kit Component
ComponentAmount
ARG2 Microplate96 Wells (12 x 8 Well strips)
ARG2 Lyophilized Standard2 x 50 ng
100X Biotinylated ARG2 Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 50 mL
Detector Antibody Diluent1 x 15 mL
Conjugate Diluent1 x 15 mL
25X Wash Buffer1 x 20 mL
Stop Solution1 x 10 mL
TMB Substrate1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates
Sensitivity0.195 ng/mL
Assay InfoAssay Methodology: Quantitative Sanadwich ELISA
Protocol Information
Gene SymbolARG2
Gene Full NameArginase-2, mitochondrial
Alias SymbolsL-arginine amidinohydrolase|L-arginine ureahydrolase|arginase 2|kidney arginase|nonhepatic arginase
NCBI Gene Id100008967
Protein NameArginase-2, mitochondrial
Description of TargetMay play a role in the regulation of extra-urea cycle arginine metabolism and also in down-regulation of nitric oxide synthesis.
Uniprot IDQ4VK78
Protein Accession #NP_001075650.1
Nucleotide Accession #NM_001082181.1
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