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Catalog No: OKCA00948
Size:96 Wells
Price: $754.00
SKU
OKCA00948
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityRat
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3.5 Hours
ELISA Kit Linearity
DilutionMean n=4 (%)Range (%)
Neat9689-101
1:29891-102
1:49485-97
1:89284-95
ELISA Kit PrincipleAviva Systems Biology ARG1 ELISA Kit (Rat) (OKCA00948) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. A antibody specific for ARG1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ARG1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample ARG1 captured in well.
ELISA Kit Range0.312 ng/mL-20 ng/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average(%)
Serum (n=5)10495-109
EDTA Plasma (n=4)10191-105
ELISA Kit ReproducibilityIntra-assay Precision: 3 samples with known low, middle and high level ARG1 were tested with 20 replicates across one plate.
Inter-assay Precision: 3 samples with known low, middle and high level ARG1 were tested on three different plates with 8 replicates, respectively.
Intra-Assay CV: <8%
Inter-Assay CV: <10%
ELISA Kit Component
ComponentAmount
ARG1 Microplate96 Wells (12 x 8 Well strips)
ARG1 Lyophilized Standard2
100X Biotinylated ARG1 Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 50 mL
Detector Antibody Diluent1 x 15 mL
Conjugate Diluent1 x 15 mL
25X Wash Buffer1 x 20 mL
Stop Solution1 x 10 mL
TMB Substrate1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates
Sensitivity0.078 ng/mL
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Protocol Information
Gene SymbolARG1
Gene Full NameArginase-1
Alias SymbolsArginase-1, Liver-type arginase, Type I arginase
NCBI Gene Id100008814
Protein NameArginase-1
Description of TargetThis protein is involved in step 1 of the subpathway that synthesizes L-ornithine and urea from L-arginine.
Uniprot IDQ95KM0
Protein Accession #NP_001075577.1
Nucleotide Accession #NM_001082108.1
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