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Catalog No: OKEH00785 (Formerly GWB-OFUMBG)
Price: $600.00
Availability: Domestic: within 2-3 weeks delivery | International: 2-3 weeks
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ARG1 ELISA Kit (Human) : 96 Wells (OKEH00785)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology ARG1 ELISA Kit (Human) (OKEH00785) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ARG1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ARG1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ARG1 captured in well.
ELISA Kit Range3.12-200ng/mL
ELISA Kit ReproducibilityMean Intra-assay CV%: < 6.1% (n = 20)
Mean Inter-assay CV%: < 9.8% (n = 20)
ELISA Kit Component
Anti-ARG1 Microplate96 Wells (12 x 8 Well strips)
ARG1 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated ARG1 Detector Antibody1 x 120 ul
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity1 ng/mL
SpecificityNatural and recombinant Human Arginase-1
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolARG1
Gene Full Namearginase 1
Alias Symbolsarginase, liver;arginase-1;liver-type arginase;type I arginase.
NCBI Gene Id383
Protein NameP05089
Description of TargetArginase-1
Uniprot ID
Protein Accession #NP_000036.2
Nucleotide Accession #NM_000045.3
Protein Size (# AA)322
Molecular Weight34 kDa
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