ARF6 Antibody - middle region (ARP54598_P050)

Catalog No: ARP54598_P050

WB Suggested Anti-ARF6 Antibody Titration: 0.2-1 ug/ml
Positive Control: MCF7 cell lysateARF6 is strongly supported by BioGPS gene expression data to be expressed in Human MCF7 cells

IHC Information: Paraffin embedded brain, cerebellum tissue, tested with an antibody Dilution of 5 ug/ml.

Host: Rabbit
Target: ARF6
Positive control (+): MCF7 Cell Lysate (N10)
Negative control (-): HeLa Cell Lysate (HL)
Antibody concentration: 1ug/ml

25 ug of the indicated Human whole cell extracts was loaded onto a 12% SDS-PAGE gel. 1 ug/mL of the antibody was used in this experiment.

Product Info

• Publications:

  Arf6 regulates the cycling and the readily releasable pool of synaptic vesicles at hippocampal synapse. Elife. 5, (2016). 26731518

  More...

• Tested Species Reactivity: Human
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Sheep, Zebrafish
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Clonality: Polyclonal
• Host: Rabbit
• Application: IHC, WB
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human ARF6
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Sheep: 100%; Zebrafish: 100%
• Peptide Sequence: Synthetic peptide located within the following region: REMRDAIILIFANKQDLPDAMKPHEIQEKLGLTRIRDRNWYVQPSCATSG
• Concentration: 0.5 mg/ml
• Blocking Peptide: For anti-ARF6 (ARP54598_P050) antibody is Catalog # AAP54598 (Previous Catalog # AAPP31382)
• Sample Type Confirmation:

  ARF6 is strongly supported by BioGPS gene expression data to be expressed in MCF7

• Enhanced Validation:

  Relative Expression (Western Blot)

Target Info

• Reference: Karim,Z.A., (2008) J. Biol. Chem. 283 (18), 11995-12003
• Gene Symbol: ARF6
• Gene Full Name: ADP-ribosylation factor 6
• Alias Symbols: DKFZp564M0264
• NCBI Gene Id: 382
• Protein Name: ADP-ribosylation factor 6
• Description of Target: ARF6 is a member of the human ARF family, which is part of the RAS superfamily. They are small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D. ARF6 is localized to the plasma membrane, and regulates vesicular trafficking, remodelling of membrane lipids, and signaling pathways that lead to actin remodeling. This gene encodes a member of the human ARF gene family, which is part of the RAS superfamily. The ARF genes encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D. The product of this gene is localized to the plasma membrane, and regulates vesicular trafficking, remodelling of membrane lipids, and signaling pathways that lead to actin remodeling. A pseudogene of this gene is located on chromosome 7. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
• Uniprot ID: P62330
• Protein Accession #: NP_001654
• Nucleotide Accession #: NM_001663
• Protein Size (# AA): 175
• Molecular Weight: 20 kDa
• Protein Interactions: UBC; LSB5; GGA1; STAU1; EGFR; CYTH1; CYTH2; GNAQ; FN1; VCP; APP; RAB11FIP3; RAB11FIP4; RAB11FIP5; USP6; IKBKG; GGA3; ITSN1; ASAP1; FBXO8; MEPCE; RUVBL2; ASAP3; MPP5; ZNF709; EXOC5; ARFIP2; AP3D1; PIP5K1C; ASAP2; AP3B1; PIP5K1A; PLD1; AP1B1; EZR; CHRM3; CA