AREG ELISA Kit (Mouse) (OKCD05604)

Catalog No: OKCD05604

Size:96 Wells

AREG ELISA Kit (Mouse) (OKCD05604) Standard Curve

Product Info

• Predicted Species Reactivity: Mouse
• Application: ELISA
• ELISA Kit Detection Method: Colorimetric, OD 450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology AREG ELISA Kit (Mouse) (OKCD05604) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for AREG has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for AREG is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample AREG captured in the well.
• ELISA Kit Range: 15.6-1,000pg/mL
• ELISA Kit Reproducibility: Intra-assay reproducibility was evaluated using 20 replicates of 3 samples representing low, middle and high level AREG. Inter-assay reproducibility was evaluated with 3 samples representing low, middle and high level AREG using 8 replicates on each of 3 plates.
  Intra-Assay CV <10%
  Inter-Assay CV <12%
• ELISA Kit Component:
  • AREG Microplate: 96 Wells (12 x 8 Well strips)
  • AREG Lyophilized Standard: 2
  • 100X AREG HRP-Detector Anitbody: 1 x 120 ÂuL
  • 100X Avidin-HRP Conjugate: 1 x 120 ÂuL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 2 mL
  • Stop Solution: 1 x 20 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Sensitivity: < 5.8pg/mL
• Publications:

  DDX3 modulates the tumor microenvironment via its role in endoplasmic reticulum-associated translation. iScience. 24, 103086 (2021). 34568799

Target Info

• Gene Symbol: AREG
• Gene Full Name: amphiregulin
• Alias Symbols: AR, Sdg, Mcub, Sdgf
• NCBI Gene Id: 11839
• Protein Name: amphiregulin
• Description of Target: This gene encodes a member of the epidermal growth factor (EGF) family and preproprotein that is proteolytically processed to generate a mature protein product. The encoded protein is a ligand of the epidermal growth factor receptor (EGFR) and has been shown to play a role in immunity, inflammation, tissue repair, and lung and mammary gland development. Homozygous knockout mice for this gene exhibit impaired immune system regulation in the skin and gene expression changes characteristic of chronic liver damage.
• Uniprot ID: P31955
• Protein Accession #: NP_033834.1
• Nucleotide Accession #: NM_009704.4