AREG ELISA Kit (Mouse) (OKCD05604)

Catalog No: OKCD05604

Size:96WELLS

AREG ELISA Kit (Mouse) (OKCD05604) Standard Curve

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Amphiregulin (AREG). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Amphiregulin (AREG). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Amphiregulin (AREG), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Amphiregulin (AREG) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 15.6-1,000pg/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Amphiregulin (AREG) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Amphiregulin (AREG) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • AREG Microplate: 96 Wells (12 x 8 Well strips)
  • AREG Lyophilized Standard: 2
  • 100X AREG HRP-Detector Anitbody: 1 x 120 ÂuL
  • 100X Avidin-HRP Conjugate: 1 x 120 ÂuL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 2 mL
  • Stop Solution: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Sensitivity: < 5.8pg/mL
• Publications:

  DDX3 modulates the tumor microenvironment via its role in endoplasmic reticulum-associated translation. iScience. 24, 103086 (2021). 34568799

• Description:
  View product citations for AREG ELISA kit (Mouse) on CiteAb

Target Info

• Gene Symbol: Areg
• Gene Full Name: amphiregulin
• Alias Symbols: amphiregulin;AR;Mcub;schwannoma-derived growth factor;Sdg;Sdgf.
• NCBI Gene Id: 11839
• Protein Name: Amphiregulin
• Description of Target: Ligand of the EGF receptor/EGFR. Autocrine growth factor as well as a mitogen for a broad range of target cells including astrocytes, Schwann cells and fibroblasts.
• Uniprot ID: P31955
• Protein Accession #: NP_033834.1
• Nucleotide Accession #: NM_009704.4