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Catalog No: OKCD05604
Size:96WELLS
Price: $475.00
SKU
OKCD05604
Availability: Domestic: within 1-2 weeks delivery | International: within 1-2 weeks delivery
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Datasheets/ManualsPrintable datasheet for AREG ELISA Kit (Mouse) (OKCD05604)
Product Info
Predicted Species ReactivityMouse|Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Amphiregulin (AREG). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Amphiregulin (AREG). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Amphiregulin (AREG), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Amphiregulin (AREG) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range15.6-1,000pg/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Amphiregulin (AREG) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Amphiregulin (AREG) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
AREG Microplate96 Wells (12 x 8 Well strips)
AREG Lyophilized Standard2
100X AREG HRP-Detector Anitbody1 x 120 ÂuL
100X Avidin-HRP Conjugate1 x 120 ÂuL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 2 mL
Stop Solution1 x 20 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity< 5.8pg/mL
Publications

DDX3 modulates the tumor microenvironment via its role in endoplasmic reticulum-associated translation. iScience. 24, 103086 (2021). 34568799

Description
Gene SymbolAreg
Gene Full Nameamphiregulin
Alias Symbolsamphiregulin;AR;Mcub;schwannoma-derived growth factor;Sdg;Sdgf.
NCBI Gene Id11839
Protein NameAmphiregulin
Description of TargetLigand of the EGF receptor/EGFR. Autocrine growth factor as well as a mitogen for a broad range of target cells including astrocytes, Schwann cells and fibroblasts.
Uniprot IDP31955
Protein Accession #NP_033834.1
Nucleotide Accession #NM_009704.4
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