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Catalog No: OKEH00009
Size:96T
Price: $500.00
SKU
OKEH00009
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology AREG ELISA Kit (Human) (OKEH00009) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for AREG has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for AREG is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample AREG captured in well.
ELISA Kit Range31.2-2000pg/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 89%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 5.8% (n = 20)
Mean Inter-assay CV%: < 8.6% (n = 20)
ELISA Kit Component
ComponentAmount
AREG Microplate96 Wells (12 x 8 Well strips)
AREG Lyophilized Standard2
100X Biotinylated AREG Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity10 pg/mL
SpecificityNatural and recombinant Human Amphiregulin
Publications

DDX3 modulates the tumor microenvironment via its role in endoplasmic reticulum-associated translation. iScience. 24, 103086 (2021). 34568799

Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Description
Gene SymbolAREG
Gene Full Nameamphiregulin
Alias Symbolsamphiregulin;amphiregulin B;AR;AREGB;colorectum cell-derived growth factor;CRDGF;schwannoma-derived growth factor;SDGF.
NCBI Gene Id374
Protein NameP15514
Description of TargetAmphiregulin
Uniprot IDhttps://www.uniprot.org/uniprot/P15514
Protein Accession #NP_001648
Nucleotide Accession #NM_001657
Protein Size (# AA)252
Molecular Weight28 kDa
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