AREG Chemi-Luminescent ELISA Kit (Mouse) (OKCD03312)

Catalog No: OKCD03312

Size:96WELLS

Mouse AREG ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Chemiluminescent immunoassay
• ELISA Kit Detection Method: Chemiluminescent
• ELISA Kit Duration: 2h, 40min
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	85-101%	80-89%	80-99%	81-96%
  EDTA Plasma (n=5)	80-101%	78-96%	97-104%	79-96%
  Heparin Plasma (n=5)	85-99%	85-96%	88-102%	82-93%

• ELISA Kit Principle: The microplate provided in this kit has been pre-coated with an antibody specific to Amphiregulin (AREG). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Amphiregulin (AREG). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Amphiregulin (AREG) level in the sample or standard.
• ELISA Kit Range: 1.37-1,000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	89-97	93
  EDTA Plasma (n=5)	94-102	98
  Heparin Plasma (n=5)	93-105	97

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Amphiregulin (AREG) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Amphiregulin (AREG) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Areg Microplate: 96 Wells (12 x 8 Well strips)
  • Areg Lyophilized Standard: 2
  • 100X Areg Biotinylated Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • 100X Luminol Substrate: 1 x 2 mL
  • Substrate Diluent: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: 15.6 pg/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Areg
• Gene Full Name: amphiregulin
• Alias Symbols: amphiregulin;AR;Mcub;schwannoma-derived growth factor;Sdg;Sdgf.
• NCBI Gene Id: 11839
• Protein Name: Amphiregulin
• Description of Target: Ligand of the EGF receptor/EGFR. Autocrine growth factor as well as a mitogen for a broad range of target cells including astrocytes, Schwann cells and fibroblasts.
• Uniprot ID: P31955
• Protein Accession #: NP_033834
• Nucleotide Accession #: NM_009704