ARAP1 Antibody (OAEE00021)

Catalog No: OAEE00021

Size:100UG

Colocalization of ARAP1 with DR4 at the plasma membrane and in early endosomes. ARAP1-transfected NCTC cells were treated with TRAIL for 0´, 30´ or 60´ and fixed with methanol. DR4 was detected by rabbit polyclonal (red) and ARAP1 with ARAP1-2 mouse monoclonal antibody (green). Arrowheads show ARAP1-DR4 colocalization at the plasma membrane and intracellular membranes, whereas arrows show their colocalization in early endosomes.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Clonality: Monoclonal
• Clone: ARAP1-2
• Isotype: IgG1
• Host: Mouse
• Application: Immunocytochemistry|Immunoprecipitation|Western blot
• Reconstitution and Storage: 2°C to 8°C
• Immunogen: His6-tagged protein corresponding to amino acids 1190-1450 of human ARAP1
• Purification: Purified
• Concentration: 1 mg/ml
• Specificity: The antibody ARAP1-2 reacts with C-terminal part of ARAP1, a 160 kDa adaptor protein.
• Formulation: Purified
• Purity: > 95% (by SDS-PAGE)
• Storage Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
• Reference: Simova S, Klima M, Cermak L, Sourkova V, Andera L: Arf and Rho GAP adapter protein ARAP1 participates in the mobilization of TRAIL-R1/DR4 to the plasma membrane. Apoptosis. 2008 Mar;13(3):423-36.
  Guo DF, Chenier I, Tardif V, Orlov SN, Inagami T: Type 1 angiotensin II receptor-associated protein ARAP1 binds and recycles the receptor to the plasma membrane. Biochem Biophys Res Commun. 2003 Oct 31;310(4):1254-65.
  Miura K, Jacques KM, Stauffer S, Kubosaki A, Zhu K, Hirsch DS, Resau J, Zheng Y, Randazzo PA: ARAP1: a point of convergence for Arf and Rho signaling. Mol Cell. 2002 Jan;9(1):109-19.

Target Info

• Gene Symbol: ARAP1
• Gene Full Name: ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 1
• Alias Symbols: arf-GAP with Rho-GAP domain, ANK repeat and PH domain-containing protein 1;ARF-GAP, RHO-GAP, ankyrin repeat, and pleckstrin homology domains-containing protein 1;centaurin-delta-2;CENTD2;cnt-d2.
• NCBI Gene Id: 116985
• Protein Name: Arf-GAP with Rho-GAP domain, ANK repeat and PH domain-containing protein 1
• Description of Target: Phosphatidylinositol 3,4,5-trisphosphate-dependent GTPase-activating protein that modulates actin cytoskeleton remodeling by regulating ARF and RHO family members. Is activated by phosphatidylinositol 3,4,5-trisphosphate (PtdIns(3,4,5)P3) binding. Can be activated by phosphatidylinositol 3,4-bisphosphate (PtdIns(3,4,5)P2) binding, albeit with lower efficiency. Has a preference for ARF1 and ARF5 (By similarity).
• Uniprot ID: Q96P48
• Protein Accession #: NP_001035207.1; NP_001128662.1; NP_056057.2
• Nucleotide Accession #: NM_001040118.2; NM_001135190.1; NM_015242.4