Arachidonic acid ELISA Kit (OKEH02583)

Catalog No: OKEH02583

Size:96 Wells

Arachidonic acid ELISA Kit (OKEH02583) standard curve.

Product Info

• Predicted Species Reactivity: All
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology Arachidonic acid ELISA Kit (OKEH02583) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Arachidonic acid antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Arachidonic acid and incubated. The Arachidonic acid found in the sample or standards competes with the biotinylated Arachidonic acid for limited binding sites on the immobilized anti-Arachidonic acid antibody. Excess unbound biotinylated Arachidonic acid and sample or standard Arachidonic acid is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Arachidonic acid captured in the well and inversely proportional to the amount of Arachidonic acid which was contained in the sample or standard.
• ELISA Kit Range: 0.78 - 50ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into Serum and Plasma = 100%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: < 5.3% (n = 20)
  Mean Inter-assay CV%: < 7.9% (n = 20)
• ELISA Kit Component:
  • Anti-Arachidonic acid Microplate: 96 Wells (12 x 8 Well strips)
  • Arachidonic acid Lyophilized Standard: 2 x 50 ng
  • Sample Diluent: 1 x 20 mL
  • 100X Arachidonic acid-Biotin Complex: 1 x 60 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Biotin Complex Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Target Abbr: AA
  Target Name: Arachidonic acid
  Pubchem: 444899
  Chemical formula: C20H32O2
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, plasma, tissue homogenates, cell culture supernatants and other biological fluids
• Sensitivity: 0.3 ng/mL
• Specificity: Natural and recombinant General Arachidonic acid
• Publications:

  Bee Venom Phospholipase A2 Ameliorates Atherosclerosis by Modulating Regulatory T Cells. Toxins (Basel). 12, NULL (2020). 32977607

  Impact of 17β-HSD12, the 3-ketoacyl-CoA reductase of long-chain fatty acid synthesis, on breast cancer cell proliferation and migration. Cell Mol Life Sci. 77, 1153-1175 (2020). 31302749

  PNPLA3 I148M mediates the regulatory effect of NF-kB on inflammation in PA-treated HepG2 cells. J Cell Mol Med. 24, 1541-1552 (2020). 31793207

  Zayed, M. et al. Diabetes adversely affects phospholipid profiles in human carotid artery endarterectomy plaques. J Lipid Res. 59(4): 730-738 (2018). PMC5880490

• Assay Info: Assay Methodology: Quantitative Competitive ELISA

Target Info

• Alias Symbols: AA, Arachidonic acid, AA
• Protein Name: Arachidonic acid
• Description of Target: Arachidonic acid is a polyunsaturated fatty acid that is present in the phospholipids (especially phosphatidylethanolamine, phosphatidylcholine, and phosphatidylinositides) of membranes of the body's cells, and is abundant in the brain, muscles, liver. Arachidonic acid (AA, sometimes ARA) is a polyunsaturated omega-6 fatty acid 20:4(ω-6). It is the counterpart to the saturated arachidic acid found in peanut oil.