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Arachidonic acid ELISA Kit (OKEH02583)

96 Wells
$559.00
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Predicted Species Reactivity:
All
Additional Information:
Target Abbr: AA
Target Name: Arachidonic acid
:::
Pubchem: 444899
:::
Chemical formula: C20H32O2
Reconstitution and Storage:
Store as indicated in product manual.
Alias Symbols:
AA, Arachidonic acid, AA
Protein Name:
Arachidonic acid
Description of Target:
Arachidonic acid is a polyunsaturated fatty acid that is present in the phospholipids (especially phosphatidylethanolamine, phosphatidylcholine, and phosphatidylinositides) of membranes of the body's cells, and is abundant in the brain, muscles, liver. Arachidonic acid (AA, sometimes ARA) is a polyunsaturated omega-6 fatty acid 20:4(ω-6). It is the counterpart to the saturated arachidic acid found in peanut oil.
Sample Type:
Serum, plasma, tissue homogenates, cell culture supernatants and other biological fluids
Sensitivity:
0.3 ng/mL
Kit Range:
0.78 - 50ng/mL
Kit Reproducibility:
Mean Intra-assay CV%: < 5.3% (n = 20)
Mean Inter-assay CV%: < 7.9% (n = 20)
Kit Duration:
~ 3 Hours
Kit Principle:
Aviva Systems Biology Arachidonic acid ELISA Kit (OKEH02583) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Arachidonic acid antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Arachidonic acid and incubated. The Arachidonic acid found in the sample or standards competes with the biotinylated Arachidonic acid for limited binding sites on the immobilized anti-Arachidonic acid antibody. Excess unbound biotinylated Arachidonic acid and sample or standard Arachidonic acid is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Arachidonic acid captured in the well and inversely proportional to the amount of Arachidonic acid which was contained in the sample or standard.
Kit Component:
ComponentAmount
Anti-Arachidonic acid Microplate96 Wells (12 x 8 Well strips)
Arachidonic acid Lyophilized Standard2 x 50 ng
Sample Diluent1 x 20 mL
100X Arachidonic acid-Biotin Complex1 x 60 uL
100X Avidin-HRP Conjugate1 x 120 uL
Biotin Complex Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Kit Recovery:
Mean recovery when spiking into Serum and Plasma = 100%
Kit Detection Method:
Colorimetric, OD450 nm
Datasheets/Manuals:
Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Specificity:
Natural and recombinant General Arachidonic acid
Publications:

Zayed, M. et al. Diabetes adversely affects phospholipid profiles in human carotid artery endarterectomy plaques. J Lipid Res. 59(4): 730-738 (2018). ELISA, PMC5880490

Assay Info:
Assay Methodology: Quantitative Competitive ELISA

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