AQUAPORIN 4 Antibody - C-terminal region (OABB01958)

Catalog No: OABB01958

Size:100ug

Anti-Aquaporin 4 antibody, OABB01958, Western blotting
All lanes: Anti Aquaporin 4 (OABB01958) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: Mouse Brain Tissue Lysate at 50ug
Predicted bind size: 35KD
Observed bind size: 45KD

Anti-Aquaporin 4 antibody, OABB01958, IHC(P)
IHC(P): Human Glioma Tissue

Anti-Aquaporin 4 antibody, OABB01958, IHC(P)
IHC(P): Mouse Brain Tissue

Anti-Aquaporin 4 antibody, OABB01958, IHC(F)
IHC(F): Mouse Brain Tissue

Anti-Aquaporin 4 antibody, OABB01958, IHC(P)
IHC(P): Rat Brain Tissue

Anti-Aquaporin 4 antibody, OABB01958, IHC(F)
IHC(F): Rat Brain Tissue

Product Info

• Tested Species Reactivity: Human, Mouse, Rat
• Product Format: Lyophilized Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg sodium azide
• Clonality: Polyclonal
• Clone: Polyclonal
• Isotype: Rabbit IgG
• Host: Rabbit
• Application: WB, IHC-P, IHC-F
• Reconstitution and Storage: Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml. At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
• Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Aquaporin 4 (295-323aa KPGVVHVIDVDRGEEKKGKDQSGEVLSSV), different from the related mouse sequence by two amino acids, and from the related rat sequence by three amino acids.
• Purification: Immunogen affinity purified.
• Specificity: No cross reactivity with other proteins.
• Application Info: WB: 0.1-0.5 ug/mL. Tested Species: Ms, Rat. Predicted Species: Hu
  IHC-P: 0.5-1 ug/mL. Tested Species: Hu, Ms, Rat. Antigen retrieval is by heat, where boiling the paraffin sections in 10mM citrate buffer (pH 6.0) for 20 mins is required for the staining of formalin/paraffin sections.
  IHC-F: 0.5-1 ug/mL. Tested Species: Ms, Rat. Predicted Species: Hu
• Publications:

  PDGF-B Is Required for Development of the Glymphatic System. Cell Rep. 26, 2955-2969.e3 (2019). 30865886

• Description: Rabbit IgG polyclonal antibody for Aquaporin-4(AQP4) detection. Tested with WB, IHC-P, IHC-F in Human;Mouse;Rat.

Target Info

• Gene Symbol: AQP4
• Gene Full Name: aquaporin 4
• Alias Symbols: AQP 4, AQP-4, AQP4, AQP4_HUMAN, Aquaporin type 4, Aquaporin-4, Aquaporin4, HMIWC 2, HMIWC2, Mercurial insensitive water channel, Mercurial-insensitive water channel, MGC22454, MIWC, WCH 4, WCH4
• NCBI Gene Id: 361
• Protein Name: Aquaporin-4
• Description of Target: Aquaporin 4 is found in the basolateral cell membrane of principal collecting duct cells and provides a pathway for water to exit these cells. The gene of AQP4 is mapped to 18q11.2-q12.1. Similar to other aquaporins, the AQP4 gene is composed of 4 exons encoding 127, 55, 27, and 92 amino acids separated by introns of 0.8, 0.3, and 5.2 kb. Unlike other aquaporins, an alternative coding initiation sequence (designated exon 0) was located 2.7 kb upstream of exon 1. When spliced together, M1 and the subsequent 10 amino acids are encoded by exon 0; the next 11 amino acids and M23 are encoded by exon 1. AQP4 is expressed in astrocytes and is upregulated by direct insult to the central nervous system. And AQP4 is the predominant water channel in the brain and has an important role in brain water homeostasis. It is abundant in mammalian brain and is concentrated in astrocytic foot processes at the blood-brain barrier.
• Uniprot ID: P55087
• Molecular Weight: 35 kDa