Catalog No: OKCD00891 (Formerly GWB-KBBLI6)
Size:96 Wells
Price: $675.00
Availability: Domestic: within 2-3 weeks delivery | International: 2-3 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHuman
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration2.5 Hours
ELISA Kit PrincipleAviva Systems Biology AQP8 ELISA Kit (Human) (OKCD00891) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for AQP8 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for AQP8 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is read by absorbance at 450 nm and is quantitatively proportional to the amount of sample AQP8captured in well.
ELISA Kit Range0.312-20 ng/mL
ELISA Kit ReproducibilityIntra-assay Precision: 3 samples with known low, middle and high level AQP8 were tested with 20 replicates across one plate.
Inter-assay Precision: 3 samples with known low, middle and high level AQP8 were tested on three different plates with 8 replicates, respectively.
Intra-Assay CV: <10%
Inter-Assay CV: <12%
ELISA Kit Component
Anti-AQP8 Microplate96 Wells (12 x 8 Well Strips)
AQP8 Lyophilized Standard2 x 20 ng
100X Biotinylated AQP8 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeTissue homogenates, cell lysates and other biological fluids
Sensitivity< 0.113 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Aquaporin 8 (AQP8). No significant cross-reactivity or interference between Aquaporin 8 (AQP8) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolAQP8
Gene Full Nameaquaporin 8
Alias SymbolsAQP-8
NCBI Gene Id343
Protein Nameaquaporin-8
Description of TargetForms a water-specific channel; mercury-sensitive. Not permeable to glycerol or urea.
Swissprot IdO94778
Protein Accession #NP_001160
Nucleotide Accession #NM_001169.2
Protein Size (# AA)261
Tissue ToolFind tissues and cell lines supported by DNA array analysis to express AQP8.
RNA SeqFind tissues and cell lines supported by RNA-seq analysis to express AQP8.
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