AQP4 Antibody - C-terminal region (OABB01083)

Catalog No: OABB01083

Size:100UG

Anti-Aquaporin 4 antibody, OABB01083, Western blotting
Lane 1: Rat Heart Tissue Lysate
Lane 2: Rat Brain Tissue Lysate
Lane 3: Rat Kidney Tissue Lysate
Lane 4: HT1080 Cell Lysate
Lane 5: MCF-7 Cell Lysate
Lane 6: COLO320 Cell Lysate

Anti-Aquaporin 4 antibody, OABB01083, IHC(P)
IHC(P): Human Lung Cancer Tissue

Anti-Aquaporin 4 antibody, OABB01083, IHC(P)
IHC(P): Rat Brain Tissue

Product Info

• Tested Species Reactivity: Human, Rat
• Predicted Species Reactivity: Hamster|Human|Mouse|Rat
• Product Format: Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
• Clonality: Polyclonal
• Clone: Polyclonal
• Isotype: Rabbit IgG
• Host: Rabbit
• Application: Immunohistochemistry|Western blot
• Additional Information: Notes: WB: The detection limit for AQP4 is approximately 0.5ng/lane under reducing conditions.
  Tested Species: In-house tested species with positive results.
  Predicted Species: Species predicted to be fit for the product based on sequence similarities.
  By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.
  Other applications have not been tested.
  Optimal dilutions should be determined by end users.
  
  Background: AQP4(Aquaporin 4), also known as MERCURIAL-INSENSITIVE WATER CHANNEL; MIWC, is protein which in humans is encoded by the AQP4 gene. The aquaporins are a family of water-selective membrane channels found in animals, plants, and microorganisms. AQP4 is the predominant water channel in the brain and has an important role in brain water homeostasis. By fluorescence in situ hybridization, Lu et al.(1996) determined that the AQP4 gene maps to 18q11.2-q12.1. By interspecific backcross analysis, Turtzo et al.(1997) mapped the mouse Aqp4 gene to the proximal region of chromosome 18. Analyzing the expression of AQP4 in mammalian skeletal muscle, Frigeri et al.(1998) found that, in immunohistochemical experiments, affinity-purified AQP4 antibodies stained selectively the sarcolemma of fast-twitch fibers. Immunocytochemistry revealed strong AQP4 water channel expression in Muller cells in mouse retina and in fibrous astrocytes in optic nerve.
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Immunogen: Polypeptide
• Purification: Affinity Purified
• Concentration: 500 ug/ml
• Specificity: No cross reactivity with other proteins.
• Application Info: Western blot: 0.1-0.5 ug/ml: Human, Rat: Mouse
  Immunohistochemistry (Paraffin-embedded Section): 0.5-1 ug/ml: Human, Rat: Mouse: By Heat
• Storage Buffer: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
• Description: Rabbit IgG polyclonal antibody for Aquaporin-4(AQP4) detection. Tested with WB, IHC-P in Human;Mouse;Rat.

Target Info

• Gene Symbol: AQP4
• Gene Full Name: aquaporin 4
• Alias Symbols: aquaporin type4;aquaporin-4;mercurial-insensitive water channel;MIWC;WCH4.
• NCBI Gene Id: 361
• Protein Name: Aquaporin-4
• Description of Target: Forms a water-specific channel. Osmoreceptor which regulates body water balance and mediates water flow within the central nervous system.
• Uniprot ID: P55087
• Molecular Weight: 34830 MW