AQP3 ELISA Kit (Human) (OKCD06536)

Catalog No: OKCD06536

Size:96WELLS

AQP3 ELISA Kit (Human) (OKCD06536) Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Aquaporin 3, Gill Blood Group (AQP3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Aquaporin 3, Gill Blood Group (AQP3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Aquaporin 3, Gill Blood Group (AQP3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Aquaporin 3, Gill Blood Group (AQP3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.78-50ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Aquaporin 3, Gill Blood Group (AQP3) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Aquaporin 3, Gill Blood Group (AQP3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • AQP3 Microplate: 96 Wells (12 x 8 Well strips)
  • AQP3 Lyophilized Standard: 2
  • 100X AQP3 HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 2 mL
  • Stop Solution: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Sensitivity: < 0.058ng/mL

Target Info

• Gene Symbol: AQP3
• Gene Full Name: aquaporin 3 (Gill blood group)
• Alias Symbols: AQP-3;aquaglyceroporin-3;aquaporin 3 (GIL blood group);aquaporin-3;GIL.
• NCBI Gene Id: 360
• Protein Name: Aquaporin-3
• Description of Target: Water channel required to promote glycerol permeability and water transport across cell membranes. Acts as a glycerol transporter in skin and plays an important role in regulating SC (stratum corneum) and epidermal glycerol content. Involved in skin hydration, wound healing, and tumorigenesis. Provides kidney medullary collecting duct with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient. Slightly permeable to urea and may function as a water and urea exit mechanism in antidiuresis in collecting duct cells. It may play an important role in gastrointestinal tract water transport and in glycerol metabolism (By similarity).
• Uniprot ID: Q92482
• Protein Accession #: NP_001305073.1
• Nucleotide Accession #: NM_001318144.1