AQP2 ELISA Kit (Human) (OKCD06534)

Catalog No: OKCD06534

Size:96WELLS

AQP2 ELISA Kit (Human) (OKCD06534) Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Aquaporin 2, Collecting Duct (AQP2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Aquaporin 2, Collecting Duct (AQP2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Aquaporin 2, Collecting Duct (AQP2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Aquaporin 2, Collecting Duct (AQP2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Aquaporin 2, Collecting Duct (AQP2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Aquaporin 2, Collecting Duct (AQP2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • AQP2 Microplate: 96 Wells (12 x 8 Well strips)
  • AQP2 Lyophilized Standard: 2
  • 100X AQP2 HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 2 mL
  • Stop Solution: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, urine and other biological fluids
• Sensitivity: < 0.117ng/mL

Target Info

• Gene Symbol: AQP2
• Gene Full Name: aquaporin 2
• Alias Symbols: ADH water channel;AQP-2;AQP-CD;aquaporin 2 (collecting duct);aquaporin-2;aquaporin-CD;collecting duct water channel protein;NDI2;water channel protein for renal collecting duct;water-channel aquaporin 2;WCH-CD.
• NCBI Gene Id: 359
• Protein Name: Aquaporin-2
• Description of Target: Forms a water-specific channel that provides the plasma membranes of renal collecting duct with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient.
• Uniprot ID: P41181
• Protein Accession #: NP_000477.1
• Nucleotide Accession #: NM_000486.5