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Catalog No: OKEH06279
Size:96T
Price: $525.00
SKU
OKEH06279
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Please click here to view the MSDS.
COA DatasheetPrintable COA datasheet for OKEH06279
Product Info
Predicted Species ReactivityRat, Rattus norvegicus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~3 Hours
ELISA Kit PrincipleAviva Systems Biology Aqp1 ELISA Kit (Rat) (OKEH06279) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Aqp1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Aqp1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Aqp1 captured in the well.
ELISA Kit Range78-5000pg/mL
ELISA Kit RecoveryMean recovery when spiking into Serum and Plasma = 92%
ELISA Kit ReproducibilityThree samples concentrations were measured in replicate within an assay plate and across replicate assays to assess Intra- and Mean Inter-Assay Precision.
Mean Intra-Assay Precision - <4.8%, n = 20
Mean Inter-Assay Precision - <7.4%, n = 20
ELISA Kit Component
ComponentAmount
AQP1 Microplate96 Wells (12 x 8 Well strips)
AQP1 Lyophilized Standard2
100X Biotinylated AQP1 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
Stop Solution1 x 10 mL
TMB Substrate1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, plasma and other biological fluids
Sensitivity39 pg/mL
SpecificityNatural and recombinant Rat Aquaporin-1
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolAqp1
Gene Full Nameaquaporin 1
Alias SymbolsAQP-1;Aquaporin 1 (aquaporin channel forming integral protein 28 (CHIP));aquaporin 1 (Colton blood group);aquaporin-1;aquaporin-CHIP;CHIP28;water channel protein for red blood cells and kidney proximal tubule.
NCBI Gene Id25240
Protein NameP29975
Description of TargetAquaporin-1
Uniprot IDhttps://www.uniprot.org/uniprot/P29975
Protein Accession #NP_036910.1
Nucleotide Accession #NM_012778.1
Protein Size (# AA)269
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