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Catalog No: OKCD06531
Size:96WELLS
Price: $600.00
SKU
OKCD06531
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Datasheets/ManualsPrintable datasheet for AQP1 ELISA Kit (Human) (OKCD06531)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Aquaporin 1 (AQP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Aquaporin 1 (AQP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Aquaporin 1 (AQP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Aquaporin 1 (AQP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.25-16ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Aquaporin 1 (AQP1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Aquaporin 1 (AQP1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
AQP1 Microplate96 Wells (12 x 8 Well strips)
AQP1 Lyophilized Standard2
100X AQP1 HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity< 0.09ng/mL
Gene SymbolAQP1
Gene Full Nameaquaporin 1 (Colton blood group)
Alias SymbolsAQP-CHIP;aquaporin 1 (channel-forming integral protein, 28kDa, CO blood group);aquaporin 1, Colton blood group antigen;aquaporin-1;aquaporin-CHIP;channel-like integral membrane protein, 28-kDa;CHIP28;CO;Colton blood group antigen;urine water channel;water channel protein for red blood cells and kidney proximal tubule.
NCBI Gene Id358
Protein NameAquaporin-1
Description of TargetForms a water-specific channel that provides the plasma membranes of red cells and kidney proximal tubules with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient.
Uniprot IDP29972
Protein Accession #NP_932766.1
Nucleotide Accession #NM_198098.3
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