Catalog No: OKBB00297
Size:96 Tests
Price: $467.00
SKU
OKBB00297
Availability: Domestic: within 1-2 week delivery | International: within 1-2 week delivery
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHuman
ApplicationELISA
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva's human APP ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A polyclonal antibody from goat specific for APP has been precoated onto 96-well plates. Standards(NS0, L18-L688) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for APP is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human APP amount of sample captured in plate.
ELISA Kit Range312 pg/ml - 20,000 pg/ml
ELISA Kit Reproducibility
Intra-Assay PrecisionInter-Assay Precision
Sample123123
Mean (ng/mL)3.376.5813.293.426.6814.21
St. Dev.0.1620.3360.7040.1980.4140.895
%CV4.805.105.295.786.196.29
ELISA Kit Component
ComponentAmount
Lyophilized recombinant human APP standard10 ng/tube x 2
Anti-human APP Antibody Well Plate96 Wells
Sample diluent buffer30 mL
Biotinylated anti-human APP antibody130 uL, dilution 1:100
Antibody diluent buffer12 mL
Avidin-Biotin-Peroxidase Complex (ABC)130 uL, dilution 1:100
ABC diluent buffer12 mL
TMB color developing agent10 mL
TMB stop solution10 mL
Additional InformationRange: 312pg/ml-20,000pg/ml
::Principle: Aviva’s human APP ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for APP has been precoated onto 96-well plates. Standards (NSO,
L18-L688) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for APP is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human APP amount of sample captured in plate.
::Notes: 1. To inspect the validity of experiment operation and the appropriateness of sample dilution proportion, pilot
experiment using standards and a small number of samples is recommended.
2. The TMB Color Developing agent is colorless and transparent before using, contact us freely if it is not the case.
3. Before using the Kit, spin tubes and bring down all components to the bottom of tubes.
4. Duplicate well assay is recommended for both standard and sample testing.
5. Don’t let 96-well plate dry, for dry plate will inactivate active components on plate.
6. Don’t reuse tips and tubes to avoid cross contamination.
7. To avoid to use the reagents from different batches together.
8. In order to avoid marginal effect of plate incubation due to temperature difference ( reaction may be stronger in the
marginal wells), it is suggested that the diluted ABC and TMB solution will be pre-warmed in 37C for 30 min before
using.
Background: Amyloid precursor protein (APP) is an integral membrane protein expressed in many tissues and concentrated in the synapses of neurons. Its primary function is not known, though it has been implicated as a regulator of synapse formation, neural plasticity and iron export. APP is best known and most commonly studied as the precursor molecule whose proteolysis generates beta amyloid (Aβ), a 39- to 42-amino acid peptide whose amyloid fibrillar form is the primary component of amyloid plaques found in the brains of Alzheimer's disease patients. APP undergoes posttranslational proteolytic processing by alpha-, beta-, and gamma-secretases. Alpha-secretase generates soluble amyloid protein, while beta- and gamma-secretases generate APP components with amyloidogenic features. These 2 processing pathways are mutually exclusive.
Reconstitution and StorageStore at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Sample Typecell culture supernates,serum and plasma (heprain, EDTA)
Sensitivity<10 pg/ml
Predicted Homology Based on Immunogen SequenceNo detectable cross-reactivity with any other cytokine
Reference1.PDB 1RW6; Wang Y, Ha Y (August 2004). "The X-ray structure of an antiparallel dimer of the human amyloid precursor
protein E2 domain". Mol. Cell 15 (3): 343–53.
2.Priller C, Bauer T, Mitteregger G, Krebs B, Kretzschmar HA, Herms J (July 2006). "Synapse formation and function is
modulated by the amyloid precursor protein". J. Neurosci. 26 (27): 7212–21.
3.Turner PR, O'Connor K, Tate WP, Abraham WC (May 2003). "Roles of amyloid precursor protein and its fragments in
regulating neural activity, plasticity and memory". Prog. Neurobiol. 70 (1): 1–32.
4.Duce JA et al. (2010). "Iron-Export Ferroxidase Activity of β- Amyloid Precursor Protein Is Inhibited by Zinc in
Alzheimer's Disease". Cell 142 (6): 857–67.
SpecificityNatural and recombinant human APP
ImmunogenExpression system for standard: NS0; Immunogen sequence: L18-L688
DescriptionFor quantitative detection of human APP in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Gene SymbolAPP
Gene Full Nameamyloid beta (A4) precursor protein
Alias SymbolsA4 amyloid protein, A4, A4_HUMAN, AAA, ABETA, ABPP, AD 1, AD1, AICD-50, AICD-57, AICD-59, AID(50), AID(57), AID(59), Alzheimer disease 1, Alzheimer disease amyloid protein, Alzheimer's disease amyloid protein, Amyloid beta (A4) precursor protein, Amyloid beta A4 protein, Amyloid beta A4 protein precursor isoform b, Amyloid beta A4 protein precursor isoform b, Amyloid beta A4 protein precursor isoform c, Amyloid beta A4 protein precursor isoform c, Amyloid beta A4 protein precursor isoform a, Amyloid beta A4 protein precursor isoform b, Amyloid beta A4 protein precursor isoform b, Amyloid beta A4 protein precursor isoform c, Amyloid beta A4 protein precursor isoform c, Amyloid beta protein, Amyloid beta-peptide, Amyloid intracellular domain 50, Amyloid intracellular domain 57, Amyloid intracellular domain 59, Amyloid of aging and alzheimer disease, APP, APP I, APPI, Beta amyloid peptide, Beta-APP40, Beta-APP42, C31, Cerebral vascular amyloid peptide, CTFgamma, CVAP, Gamma-CTF(50), Gamma-CTF(57), Gamma-CTF(59), Human mRNA for amyloid A4 precursor of Alzheimer's disease, peptidase nexin II, PN 2, PN II, PN-II, PN2, PreA4, Protease nexin II, Protease nexin-II, S-APP-alpha, S-APP-beta
NCBI Gene Id351
Protein NameAmyloid beta A4 protein
Description of TargetAmyloid precursor protein (APP) is an integral membrane protein expressed in many tissues and concentrated in the synapses of neurons. Its primary function is not known, though it has been implicated as a regulator of synapse formation, neural plasticity and iron export. APP is best known and most commonly studied as the precursor molecule whose proteolysis generates beta amyloid (Aβ), a 39- to 42-amino acid peptide whose amyloid fibrillar form is the primary component of amyloid plaques found in the brains of Alzheimer's disease patients. APP undergoes posttranslational proteolytic processing by alpha-, beta-, and gamma-secretases. Alpha-secretase generates soluble amyloid protein, while beta- and gamma-secretases generate APP components with amyloidogenic features. These 2 processing pathways are mutually exclusive.
Uniprot IDP05067
Protein Accession #NP_000475.1
Nucleotide Accession #NM_000484.3
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