Catalog No: OKBB00297
Size:96 Tests
Price: $467.00
SKU
OKBB00297
Availability: Domestic: within 1-2 week delivery | International: within 1-2 week delivery
Contact Us:
- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
Shipping Info:
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
Datasheets/Manuals | Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit. |
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Predicted Species Reactivity | Human | |||||||||||||||||||||||||||||||||||
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Application | ELISA | |||||||||||||||||||||||||||||||||||
ELISA Kit Detection Method | Colorimetric, OD450 nm | |||||||||||||||||||||||||||||||||||
ELISA Kit Duration | ~ 3 Hours | |||||||||||||||||||||||||||||||||||
ELISA Kit Principle | Aviva's human APP ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A polyclonal antibody from goat specific for APP has been precoated onto 96-well plates. Standards(NS0, L18-L688) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for APP is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human APP amount of sample captured in plate. | |||||||||||||||||||||||||||||||||||
ELISA Kit Range | 312 pg/ml - 20,000 pg/ml | |||||||||||||||||||||||||||||||||||
ELISA Kit Reproducibility |
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ELISA Kit Component |
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Additional Information | Range: 312pg/ml-20,000pg/ml | |||||||||||||||||||||||||||||||||||
:: | Principle: Aviva’s human APP ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for APP has been precoated onto 96-well plates. Standards (NSO, L18-L688) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for APP is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human APP amount of sample captured in plate. | |||||||||||||||||||||||||||||||||||
:: | Notes: 1. To inspect the validity of experiment operation and the appropriateness of sample dilution proportion, pilot experiment using standards and a small number of samples is recommended. 2. The TMB Color Developing agent is colorless and transparent before using, contact us freely if it is not the case. 3. Before using the Kit, spin tubes and bring down all components to the bottom of tubes. 4. Duplicate well assay is recommended for both standard and sample testing. 5. Don’t let 96-well plate dry, for dry plate will inactivate active components on plate. 6. Don’t reuse tips and tubes to avoid cross contamination. 7. To avoid to use the reagents from different batches together. 8. In order to avoid marginal effect of plate incubation due to temperature difference ( reaction may be stronger in the marginal wells), it is suggested that the diluted ABC and TMB solution will be pre-warmed in 37C for 30 min before using. Background: Amyloid precursor protein (APP) is an integral membrane protein expressed in many tissues and concentrated in the synapses of neurons. Its primary function is not known, though it has been implicated as a regulator of synapse formation, neural plasticity and iron export. APP is best known and most commonly studied as the precursor molecule whose proteolysis generates beta amyloid (Aβ), a 39- to 42-amino acid peptide whose amyloid fibrillar form is the primary component of amyloid plaques found in the brains of Alzheimer's disease patients. APP undergoes posttranslational proteolytic processing by alpha-, beta-, and gamma-secretases. Alpha-secretase generates soluble amyloid protein, while beta- and gamma-secretases generate APP components with amyloidogenic features. These 2 processing pathways are mutually exclusive. | |||||||||||||||||||||||||||||||||||
Reconstitution and Storage | Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.) | |||||||||||||||||||||||||||||||||||
Sample Type | cell culture supernates,serum and plasma (heprain, EDTA) | |||||||||||||||||||||||||||||||||||
Sensitivity | <10 pg/ml | |||||||||||||||||||||||||||||||||||
Predicted Homology Based on Immunogen Sequence | No detectable cross-reactivity with any other cytokine | |||||||||||||||||||||||||||||||||||
Reference | 1.PDB 1RW6; Wang Y, Ha Y (August 2004). "The X-ray structure of an antiparallel dimer of the human amyloid precursor protein E2 domain". Mol. Cell 15 (3): 343–53. 2.Priller C, Bauer T, Mitteregger G, Krebs B, Kretzschmar HA, Herms J (July 2006). "Synapse formation and function is modulated by the amyloid precursor protein". J. Neurosci. 26 (27): 7212–21. 3.Turner PR, O'Connor K, Tate WP, Abraham WC (May 2003). "Roles of amyloid precursor protein and its fragments in regulating neural activity, plasticity and memory". Prog. Neurobiol. 70 (1): 1–32. 4.Duce JA et al. (2010). "Iron-Export Ferroxidase Activity of β- Amyloid Precursor Protein Is Inhibited by Zinc in Alzheimer's Disease". Cell 142 (6): 857–67. | |||||||||||||||||||||||||||||||||||
Specificity | Natural and recombinant human APP | |||||||||||||||||||||||||||||||||||
Immunogen | Expression system for standard: NS0; Immunogen sequence: L18-L688 | |||||||||||||||||||||||||||||||||||
Description | For quantitative detection of human APP in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
Gene Symbol | APP |
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Gene Full Name | amyloid beta (A4) precursor protein |
Alias Symbols | A4 amyloid protein, A4, A4_HUMAN, AAA, ABETA, ABPP, AD 1, AD1, AICD-50, AICD-57, AICD-59, AID(50), AID(57), AID(59), Alzheimer disease 1, Alzheimer disease amyloid protein, Alzheimer's disease amyloid protein, Amyloid beta (A4) precursor protein, Amyloid beta A4 protein, Amyloid beta A4 protein precursor isoform b, Amyloid beta A4 protein precursor isoform b, Amyloid beta A4 protein precursor isoform c, Amyloid beta A4 protein precursor isoform c, Amyloid beta A4 protein precursor isoform a, Amyloid beta A4 protein precursor isoform b, Amyloid beta A4 protein precursor isoform b, Amyloid beta A4 protein precursor isoform c, Amyloid beta A4 protein precursor isoform c, Amyloid beta protein, Amyloid beta-peptide, Amyloid intracellular domain 50, Amyloid intracellular domain 57, Amyloid intracellular domain 59, Amyloid of aging and alzheimer disease, APP, APP I, APPI, Beta amyloid peptide, Beta-APP40, Beta-APP42, C31, Cerebral vascular amyloid peptide, CTFgamma, CVAP, Gamma-CTF(50), Gamma-CTF(57), Gamma-CTF(59), Human mRNA for amyloid A4 precursor of Alzheimer's disease, peptidase nexin II, PN 2, PN II, PN-II, PN2, PreA4, Protease nexin II, Protease nexin-II, S-APP-alpha, S-APP-beta |
NCBI Gene Id | 351 |
Protein Name | Amyloid beta A4 protein |
Description of Target | Amyloid precursor protein (APP) is an integral membrane protein expressed in many tissues and concentrated in the synapses of neurons. Its primary function is not known, though it has been implicated as a regulator of synapse formation, neural plasticity and iron export. APP is best known and most commonly studied as the precursor molecule whose proteolysis generates beta amyloid (Aβ), a 39- to 42-amino acid peptide whose amyloid fibrillar form is the primary component of amyloid plaques found in the brains of Alzheimer's disease patients. APP undergoes posttranslational proteolytic processing by alpha-, beta-, and gamma-secretases. Alpha-secretase generates soluble amyloid protein, while beta- and gamma-secretases generate APP components with amyloidogenic features. These 2 processing pathways are mutually exclusive. |
Uniprot ID | P05067 |
Protein Accession # | NP_000475.1 |
Nucleotide Accession # | NM_000484.3 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
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