Catalog No: OOSB00002
Size:100Tests
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Datasheets/Manuals | Printable datasheet for OOSB00002 |
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Product Format | Propidium Iodide (PI) |
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Application | FC |
Additional Information | Description: Propidium Iodide (PI) is a ready-to-use cell viability dye for the exclusion of nonviable cells in flow cytometry analysis. The solution can be used in conjunction with fluorescein (FITC), cyanine-5 (CY5), and allophycocyanin (APC) conjugated Annexin V to distinguish cells that are in the early versus late stages of apoptosis. Excitation/emission û 536/617 nm |
Reconstitution and Storage | Store at 2-8C |
Concentration | Lot specific |
Application Info | Flow cytometry analysis of viable cells. Product can be used as a vital dye in conjunction with the following Aviva Annexin V conjugates: 10040-02 Annexin V-FITC 10040-22 Annexin V-FLMA 10040-15 Annexin V-CYA5 10040-20 Annexin V-CYA5.5. |
Storage | - Store at 2- 8 C. Protect product from light. |
Warning | PI is a potential carcinogen. It is recommended that the user wear protective clothing, gloves, andeye/face protection. Avoid contact with skin and eyes. Reagent contains sodium azide. Sodium azide isvery toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protectionwhen handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled,contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Diluteazide- containing compounds in running water before discarding to avoid accumulation of potentiallyexplosive deposits in lead or copper plumbing. |
Dilution | 10 uL/106 cells |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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