Catalog No: OPSB00053
Price: $269.00
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsPrintable datasheet for OPSB00053
Product Info
HostExpressed in E. coli
ConjugationCY5: Cyanine 5
ApplicationFC, IHC, ICC
Additional InformationDescription: ApoScreen Annexin V is a recombinant protein that is expressed in E. coli. A number of changes in cell structure occur during apoptosis. One major change is the loss of membrane phospholipid asymmetry, with translocation of phosphatidylserine (PS) from the inner leaflet of the phospholipid bilayer to the external surface. Annexin V, a member of the annexin family of calcium-dependent phospholipid binding proteins, has a high affinity for phosphatidylserine (PS)-containing phospholipid bilayers. Conjugated Annexin V can be used to monitor changes in cell membrane phospholipid asymmetry, thereby providing a convenient tool for detection of apoptotic cells.
Reconstitution and StorageStore at 2-8C
DilutionFlow Cytometry: Fluorescein conjugates
Biotin conjugate
R-phycoerythrin conjugate
Allophycocyanin conjugate
Cyanine 5 conjugate
10 ul/106 cells
10 ul/106 cells
10 ul/106 cells
10 ul/106 cells
ConcentrationLot specific
CharacterizationTo insure acceptable performance, each batch of product is tested by flow cytometry to conform to thecharacteristics of a standard reference reagent.
Storage- Annexin V- UNLB: Lyophilized material. Dissolve in 1.0mL of dH2O. Store reconstituted material at 2- 8 C
- Annexin V- FITC: Store at 2- 8 C; protect from light
- Annexin V- BIOT: Store at 2- 8 C
- Annexin V- R- PE: Store at 2- 8 C; protect from light
- Annexin V- APC: Store at 2- 8 C; protect from light
- Annexin V- CY 5: Store at 2- 8 C; protect from light
- Annexin V- FLMA: Store at 2- 8 C; protect from light
- Reagents are stable for the period shown on the label if stored as directed.
Application InfoBlocking Annexin V-conjugates, Flow cytometry, Fluorescence microscopy
Other Applications DataSince applications vary, you should determine the optimum workingamount of the product that is appropriate for your specific need.
Reference1. Fadok, V.A. et al. 1992. J. Immunol. 148:22072. Tait, J.F., et al. 1989. J. Biol. Chem. 264:7944
3. Andree, H.A., et al. 1990. J. Biol. Chem. 265:4923
4. Thiagarajan, P., et al. 1990. J. Biol. Chem. 265:17420
5. Dachary, P.J., et al. 1993. Blood 81:2554
6. Koopman, G., et al. 1994. Blood 84:1415
7. Martin, S.J., et al. 1995. J. Exp. Med. 182:1545
8. Wood, B.L., et al. 1996. Blood 88:1873.
WarningReagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin,eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash withcopious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxichydrazoic acid under acidic conditions. Dilute azide- containing compounds in running water before discardingto avoid accumulation of potentially explosive deposits in lead or copper plumbing.
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