Aposcreen Annexin V Protein : CY5 (OPSB00053)

Catalog No: OPSB00053

Size:100Tests

Day old BALB/c mouse bone marrow cells were stained with Annexin V-CY5 with and without Propidium Iodide.

Amount Used: 10 uL/106 cells Two-color flow cytometry analysis of untreated (left panel) and irradiated (right panel) BALB/c thymocytes stained with annexin V-FITC and propidium iodide. Early apoptotic cells are located in the lower right quadrant of the two displays.

Product Info

• Host: Expressed in E. coli
• Conjugation: CY5: Cyanine 5
• Application: FC, IHC, ICC
• Additional Information: Description: ApoScreen Annexin V is a recombinant protein that is expressed in E. coli. A number of changes in cell structure occur during apoptosis. One major change is the loss of membrane phospholipid asymmetry, with translocation of phosphatidylserine (PS) from the inner leaflet of the phospholipid bilayer to the external surface. Annexin V, a member of the annexin family of calcium-dependent phospholipid binding proteins, has a high affinity for phosphatidylserine (PS)-containing phospholipid bilayers. Conjugated Annexin V can be used to monitor changes in cell membrane phospholipid asymmetry, thereby providing a convenient tool for detection of apoptotic cells.
• Reconstitution and Storage: Store at 2-8C
• Dilution: Flow Cytometry: Fluorescein conjugates
  Biotin conjugate
  R-phycoerythrin conjugate
  Allophycocyanin conjugate
  Cyanine 5 conjugate
  10 ul/10⁶ cells
  10 ul/10⁶ cells
  10 ul/10⁶ cells
  10 ul/10⁶ cells
• Concentration: Lot specific
• Characterization: To insure acceptable performance, each batch of product is tested by flow cytometry to conform to thecharacteristics of a standard reference reagent.
• Storage: - Annexin V- UNLB: Lyophilized material. Dissolve in 1.0mL of dH2O. Store reconstituted material at 2- 8 C
  - Annexin V- FITC: Store at 2- 8 C; protect from light
  - Annexin V- BIOT: Store at 2- 8 C
  - Annexin V- R- PE: Store at 2- 8 C; protect from light
  - Annexin V- APC: Store at 2- 8 C; protect from light
  - Annexin V- CY 5: Store at 2- 8 C; protect from light
  - Annexin V- FLMA: Store at 2- 8 C; protect from light
  - Reagents are stable for the period shown on the label if stored as directed.
• Application Info: Blocking Annexin V-conjugates, Flow cytometry, Fluorescence microscopy
• Other Applications Data: Since applications vary, you should determine the optimum workingamount of the product that is appropriate for your specific need.
• Reference: 1. Fadok, V.A. et al. 1992. J. Immunol. 148:22072. Tait, J.F., et al. 1989. J. Biol. Chem. 264:7944
  3. Andree, H.A., et al. 1990. J. Biol. Chem. 265:4923
  4. Thiagarajan, P., et al. 1990. J. Biol. Chem. 265:17420
  5. Dachary, P.J., et al. 1993. Blood 81:2554
  6. Koopman, G., et al. 1994. Blood 84:1415
  7. Martin, S.J., et al. 1995. J. Exp. Med. 182:1545
  8. Wood, B.L., et al. 1996. Blood 88:1873.
• Warning: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin,eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash withcopious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxichydrazoic acid under acidic conditions. Dilute azide- containing compounds in running water before discardingto avoid accumulation of potentially explosive deposits in lead or copper plumbing.