Aviva Systems Biology will be closed in observance of Good Friday 3/29/2024. If you are in need of any assistance please email info@avivasysbio.com

Catalog No: OKSB00007
Size:100T
Price: $399.00
SKU
OKSB00007
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
Bulk
Order
Aviva's
Satisfaction
Guarantee
Contact Us:
Shipping Info:
  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
  • Contact us for international orders.
Datasheets/ManualsPrintable datasheet for Aposcreen Annexin V Apoptosis Kit : FITC (OKSB00007)
Product Info
HostExpressed in E. coli
ConjugationFITC
ApplicationFC, ICC
ELISA Kit Component1. Annexin V-FITC is supplied as 100 tests in 1.0 mL of Annexin V Binding Buffer.
2. Propidium Iodide (PI) is supplied as 100 tests in 1.0 mL of Annexin V Binding Buffer.
3. Annexin V Buffer 10X is supplied as 7 x 1.5 mL.
Additional InformationDescription: ApoScreen Annexin V Apoptosis Kit-FITC employs a fluorescein-conjugated Annexin V (Annexin V-FITC) in concert with propidium iodide (PI) to evaluate subpopulations of cells undergoing apoptosis. During the early stages of apoptosis, cells begin to display phosphatidylserine (PS) on the outer cell membrane where it is readily detectable by staining the cells with Annexin V-FITC. As the plasma membrane becomes increasingly permeable during the later stages of apoptosis, PI can readily move across the cell membrane and bind to cellular DNA, providing a means for identifying those cells that have lost membrane integrity through mechanisms including necrosis. When cells are double stained with Annexin V-FITC and PI, three different cell populations may be observed - (i) live cells that do not stain with either Annexin V-FITC or PI; (ii) necrotic cells that stain with both reagents; and (iii) apoptotic cells that stain with Annexin V-FITC only. Analysis may be performed on any flow cytometer equipped with a single laser using excitation at 488 nm. Alternatively, the cells can be examined with a fluorescence microscope equipped with filters for discrimination of FITC and rhodamine/PI.
Reconstitution and StorageStore at 2-8C
Application Kit- 1 mL Annexin V- FITC: Store at 2- 8 C. Use as directed; no pre- dilution necessary
- 1 mL propidium iodide: Store at 2- 8 C. Use as directed; no pre- dilution necessary. Avoid skin contact and dispose ofproperly
- 7 x 1.5 mL 10X binding buffer: Store at 2- 8 C. Dilute 1 part 10X buffer with 9 parts dH20 prior to use
Note: Annexin V is produced by Aviva Systems Biology
Reference1. Cohen, J.J. 1991. Adv. Immunol. 50:55
2. MacDonald, HR, and R.K. Lees. 1990. Nature 343:642
3. Liu, Y.J., et al. 1989. Nature 342:929
4. Arends, M.J., and A.H. Wylie. 1991. Int. Rev. Exp. Pathol. 32:223
5. Wylie, A.H., et al. 1991. J. Pathol. 142:67
6. Fadok, V.A., et al. 1992. J. Immunol. 148:22077. Tait, J.F., et al. 1989. J. Biol. Chem. 264:7944
8. Andree, H.A., et al. 1990. J. Biol. Chem. 265:4923
9. Thiagarajan, P., et al. 1990. J. Biol. Chem. 265:17420
10. Dachary, P.J., et al. 1993. Blood 81:2554
11. Koopman, G., et al. 1994. Blood 84:1415
12. Martin,S.J., et al. 1995. J. Exp. Med. 182:1545
13. Wood, B.L., et al. 1996. Blood 88:1873
14. Gavriell, Y., et al. 1992. J. Cell. Biol. 119:493.
Application InfoAnnexin V-FITC: 10 uL/10^6 cells Propidium Iodide (PI): 10 uL/10^6 cells Annexin Binding Buffer 10X: Dilute 1 part 10X buffer with 9 parts dH2O just prior to use For flow cytometry, the suggested use of these reagents is in a final volume of 100 uL of Annexin Binding Buffer 1X.
Protocol Information1. Wash cells twice in cold PBS.
2. Resuspend cells in cold 1X Annexin Binding Buffer to a concentration of 1 x 106 cells/mL.
3. Add 100 uL of cell suspension (106) to 5 mL polystyrene round bottom tubes.
4. Add 10 uL of conjugated Annexin V-FITC.
5. Gently vortex each tube and incubate for 15 minutes at 2-8C and protected from light.
6. Without washing, add ~380 uL of cold 1X Annexin Binding Buffer to each tube.
7. Add 10 uL of Propidium Iodide (PI).
8. Analyze by flow cytometry immediately.
Write Your Own Review
You're reviewing:Aposcreen Annexin V Apoptosis Kit : FITC (OKSB00007)
Your Rating