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Catalog No: OKCA02338
Size:96 Wells
Price: $754.00
SKU
OKCA02338
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityMouse
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3.5 Hours
ELISA Kit Linearity
DilutionMean Recovery (%)Range (%)
1:10008681-92
1:20009588-99
1:40009386-98
1:800010096-107
ELISA Kit PrincipleAviva Systems Biology Apom ELISA Kit (Mouse) (OKCA02338) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Apom has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Apom is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample or standard Apom captured in the well.
ELISA Kit Range1.56 - 100 ng/mL
ELISA Kit Recovery
Sample TypeMean Recovery (%)Range (%)
Serum (n=5)9488-99
EDTA Plasma (n=4)9994-106
ELISA Kit ReproducibilityMean Intra-Assay: CV <12% (n=20)
Mean Inter-Assay: CV <15% (n=24)
ELISA Kit Component
ComponentAmount
Apom Microplate96 Wells (12 x 8 Well strips)
Apom Lyophilized Standard2
100X Biotinylated Apom Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 50 mL
Detector Antibody Diluent1 x 15 mL
Conjugate Diluent1 x 15 mL
25X Wash Buffer1 x 20 mL
Stop Solution1 x 10 mL
TMB Substrate1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates
Sensitivity0.39 ng/mL
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolAPOM
Alias SymbolsG3, G3a, NG2, NG20, 1190010O19Rik
NCBI Gene Id55938
Protein NameApolipoprotein M
Description of TargetProbably involved in lipid transport. Can bind sphingosine-1-phosphate, myristic acid, palmitic acid and stearic acid, retinol, all-trans-retinoic acid and 9-cis-retinoic acid.
Uniprot IDQ9Z1R3
Protein Accession #NP_061286
Nucleotide Accession #NM_018816
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