APOLIPOPROTEIN E Antibody (OASA11404)

Catalog No: OASA11404 (Formerly GWB-Q00976)

Size:100UG

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid. Phosphate buffered saline
• Clonality: Monoclonal
• Isotype: IgG1
• Host: Mouse
• Application: Enzyme-linked immunosorbent assay|Western blot
• Additional Information: Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
  Preservative Stabilisers: 0.09% Sodium Azide (NaN3)
  Buffer Solutions: Phosphate buffered saline
• Reconstitution and Storage: -20°C
• Immunogen: Purified ApoHDL fraction.
• Purification: Protein G Purified
• Predicted Homology Based on Immunogen Sequence: Human
• Concentration: 1 mg/ml
• Specificity: OASA11404 specifically recognises an epitope within amino acids 140-160 of human apolipoprotein E (Apo-E), a major component of very low-density lipoproteins (VLDLs). Apo-E is the principle apolipoprotein in the central nervous system, and is secreted by most organs into the plasma, playing a vital role in the binding, internalization and catabolism of triglyceride-rich lipoprotein constituents.
  Apo-E acts as a ligand for both the specific apo-E receptor (chylomicron remnant) of hepatic tissues, and the apoB,E (LDL) receptor. Three isoforms of Apo-E have been identified, ApoE2, E3 and E4, and have been linked with various disorders. ApoE2 has been shown to bind LPL receptors with low affinity, resulting in increased plasma cholesterol and triglyceride levels, and thereby an increased risk in cardiovascular disorders. ApoE4 is a known high risk factor for Alzheimer's disease, and in particular late onset Alzheimer disease 2 (AD2), whilst ApoE3 is the most common isoform, and considered the normal/natural Apo-E genotype.
  Clone WUE-4 has been shown to inhibit Apo-E mediated binding of lipoproteins to the apoB,E cell receptor, see Krul, E.S et al for details. Aviva recommends the use of OASA11403 for functional studies.
• Application Info: Western Blotting: OASA11404 detects a major band of approximately 34-36kDa in human liver cell lysates.
  ELISA: 1/100 - 1/1000

Target Info

• Alias Symbols: AD2, LPG, APO-E, ApoE4, LDLCQ5
• NCBI Gene Id: 348
• Protein Name: Apolipoprotein E
• Uniprot ID: P02649
• Protein Accession #: NP_000032.1