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Catalog No: OKCD00393 (Formerly GWB-KBBVA4)
Size:96WELLS
Price: $675.00
SKU
OKCD00393
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Datasheets/ManualsPrintable datasheet for APOL2 ELISA Kit (Human) (OKCD00393)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
serum(n=5)92-102%79-95%98-105%94-101%
EDTA plasma(n=5)96-103%79-96%80-102%87-104%
heparin plasma(n=5)80-101%78-92%87-102%94-103%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Apolipoprotein L2 (APOL2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Apolipoprotein L2 (APOL2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Apolipoprotein L2 (APOL2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Apolipoprotein L2 (APOL2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
serum(n=5)89-10198
EDTA plasma(n=5)87-9995
heparin plasma(n=5)96-10599
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Apolipoprotein L2 (APOL2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Apolipoprotein L2 (APOL2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-APOL2 Microplate96 Wells (12 x 8 Well Strips)
APOL2 Lyophilized Standard2 x 10 ng
100X Biotinylated APOL2 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma and other biological fluids.
Sensitivity< 0.067 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Apolipoprotein L2 (APOL2). No significant cross-reactivity or interference between Apolipoprotein L2 (APOL2) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolAPOL2
Gene Full Nameapolipoprotein L2
Alias SymbolsAPOL3;APOL-II;apolipoprotein L, 2;apolipoprotein L2;apolipoprotein L-II.
NCBI Gene Id23780
Protein NameApolipoprotein L2
Description of TargetMay affect the movement of lipids in the cytoplasm or allow the binding of lipids to organelles.
Uniprot IDQ9BQE5
Protein Accession #NP_112092
Nucleotide Accession #NM_030882.3
Protein Size (# AA)337
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