Catalog No: OKEH04368
Price: $525.00
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
COA DatasheetPrintable COA datasheet for OKEH04368
Product Info
Predicted Species ReactivityMouse, Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology Apoe ELISA Kit (Mouse) (OKEH04368) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Apoe has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for Apoe is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Apoe captured in the well.
ELISA Kit Range7.8-500ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 103%
ELISA Kit ReproducibilityMean Intra-assay CV%: <= 4.6% (n = 20)
Mean Inter-assay CV%: <= 8.4% (n = 20)
ELISA Kit Component
Anti-Apoe Microplate96 Wells (12 x 8 Well Strips)
Apoe Lyophilized Standard2 x 2 ng
Sample Diluent1 x 20 mL
Apoe Biotinylated Detector Antibody1 x 120 uL
Detector Antibody Diluent1 x 12 mL
HRP Avidin Conjugate1 x 120 uL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity3.96 ng/mL
SpecificityNatural and recombinant Mouse Apolipoprotein E

Toll-like Receptor 4-Independent Effects of Lipopolysaccharide Identified Using Longitudinal Serum Proteomics. J Proteome Res. 19, 1258-1266 (2020). 32037835

Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolApoe
Gene Full Nameapolipoprotein E
Alias SymbolsA;AI255918;Apo-E;apolipoprotein E.
NCBI Gene Id11816
Protein NameP08226
Description of TargetApolipoprotein E
Uniprot IDhttps://www.uniprot.org/uniprot/P08226
Protein Accession #NP_001292748.1
Nucleotide Accession #NM_001305819.1
Protein Size (# AA)311
Molecular Weight36 KDa
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