APOE Chemi-Luminescent ELISA Kit (Human) (OKCD03306)

Catalog No: OKCD03306

Size:96WELLS

Human APOE ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Chemiluminescent immunoassay
• ELISA Kit Detection Method: Chemiluminescent
• ELISA Kit Duration: 2h, 40min
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	88-101%	91-99%	99-105%	84-102%
  EDTA Plasma (n=5)	86-99%	87-104%	96-104%	87-97%
  Heparin Plasma (n=5)	85-103%	91-101%	82-101%	91-98%

• ELISA Kit Principle: The microplate provided in this kit has been pre-coated with an antibody specific to Apolipoprotein E (APOE). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Apolipoprotein E (APOE). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Apolipoprotein E (APOE) level in the sample or standard.
• ELISA Kit Range: 0.82-600ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	97-105	101
  EDTA Plasma (n=5)	98-105	102
  Heparin Plasma (n=5)	85-103	90

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Apolipoprotein E (APOE) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Apolipoprotein E (APOE) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • APOE Microplate: 96 Wells (12 x 8 Well strips)
  • APOE Lyophilized Standard: 2
  • 100X APOE Biotinylated Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • 100X Luminol Substrate: 1 x 2 mL
  • Substrate Diluent: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Sensitivity: 0.82 ng/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: APOE
• Gene Full Name: apolipoprotein E
• Alias Symbols: AD2;APO-E;ApoE4;apolipoprotein E;apolipoprotein E3;LDLCQ5;LPG.
• NCBI Gene Id: 348
• Protein Name: Apolipoprotein E
• Description of Target: Mediates the binding, internalization, and catabolism of lipoprotein particles. It can serve as a ligand for the LDL (apo B/E) receptor and for the specific apo-E receptor (chylomicron remnant) of hepatic tissues.
• Uniprot ID: P02649
• Protein Accession #: NP_000032
• Nucleotide Accession #: NM_000041