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Catalog No: OKCD00850 (Formerly GWB-KBBGW4)
Size:96WELLS
Price: $650.00
SKU
OKCD00850
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Datasheets/ManualsPrintable datasheet for APOC3 ELISA Kit (Mouse) (OKCD00850)
Product Info
Predicted Species ReactivityMouse|Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
serum(n=5)85-92%84-93%95-103%89-97%
EDTA plasma(n=5)94-101%99-105%98-105%80-104%
heparin plasma(n=5)94-101%91-98%82-94%80-90%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Apolipoprotein C3 (APOC3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Apolipoprotein C3 (APOC3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Apolipoprotein C3 (APOC3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Apolipoprotein C3 (APOC3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.78-50ng/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
serum(n=5)94-10298
EDTA plasma(n=5)80-9988
heparin plasma(n=5)97-105101
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Apolipoprotein C3 (APOC3) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Apolipoprotein C3 (APOC3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-Apoc3 Microplate96 Wells (12 x 8 Well Strips)
Apoc3 Lyophilized Standard2 x 20 ng
100X Biotinylated Apoc3 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity< 0.127 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Apolipoprotein C3 (APOC3). No significant cross-reactivity or interference between Apolipoprotein C3 (APOC3) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolApoc3
Gene Full Nameapolipoprotein C-III
Alias SymbolsapoC-III;apo-CIII;apolipoprotein C3;apolipoprotein C-III.
NCBI Gene Id11814
Protein NameApolipoprotein C-III
Description of TargetComponent of triglyceride-rich very low density lipoproteins (VLDL) and high density lipoproteins (HDL) in plasma. Plays a multifaceted role in triglyceride homeostasis. Intracellularly, promotes hepatic very low density lipoprotein 1 (VLDL1) assembly and secretion; extracellularly, attenuates hydrolysis and clearance of triglyceride-rich lipoproteins (TRLs). Impairs the lipolysis of TRLs by inhibiting lipoprotein lipase and the hepatic uptake of TRLs by remnant receptors. Formed of several curved helices connected via semiflexible hinges, so that it can wrap tightly around the curved micelle surface and easily adapt to the different diameters of its natural binding partners.
Uniprot IDP33622
Protein Accession #NP_001276685
Nucleotide Accession #NM_001289756.1
Protein Size (# AA)99
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