Apoc2 ELISA Kit (Mouse) (OKEH05270)

Catalog No: OKEH05270

Size:96 Wells

Mouse Apoc2 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Mouse
• ELISA Kit Detection Method: Colorimetric, OD 450 nm
• ELISA Kit Duration: ~3 Hours
• ELISA Kit Principle: Aviva Systems Biology Apoc2 ELISA Kit (Mouse) (OKEH05270) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Apoc2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Apoc2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Apoc2 captured in the well.
• ELISA Kit Range: 0.312 - 20ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into Serum and Plasma = 102.1%
• ELISA Kit Reproducibility: Three samples concentrations were measured in replicate within an assay plate and across replicate assays to assess Intra- and Mean Inter-Assay Precision.
  Mean Intra-Assay Precision - <6.8%, n = 20
  Mean Inter-Assay Precision - <9.4%, n = 20
• ELISA Kit Component:
  • Apoc2 Microplate: 96 Wells (12 x 8 Well strips)
  • Apoc2 Lyophilized Standard: 2
  • 100X Biotinylated Apoc2 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • Stop Solution: 1 x 10 mL
  • TMB Substrate: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: 0.156 ng/mL
• Specificity: Natural and recombinant Mouse Apolipoprotein C-II
• Publications:

  CREBH normalizes dyslipidemia and halts atherosclerosis in diabetes by decreasing circulating remnant lipoproteins. J Clin Invest. 131 (2021). 34491909

• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Apoc2
• Gene Full Name: Apolipoprotein C-II
• Alias Symbols: apo-CII, apoC-II
• NCBI Gene Id: 11813
• Protein Name: Apolipoprotein C-II
• Description of Target: Component of chylomicrons, very low-density lipoproteins (VLDL), low-density lipoproteins (LDL), and high-density lipoproteins (HDL) in plasma. Plays an important role in lipoprotein metabolism as an activator of lipoprotein lipase.
• Uniprot ID: Q05020
• Protein Accession #: NP_001264873.1
• Nucleotide Accession #: NM_001277944.1
• Protein Size (# AA): 97