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Catalog No: OKEH05270
Size:96T
Price: $525.00
SKU
OKEH05270
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
COA DatasheetPrintable COA datasheet for OKEH05270
Product Info
Predicted Species ReactivityMouse, Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD 450 nm
ELISA Kit Duration~3 Hours
ELISA Kit PrincipleAviva Systems Biology Apoc2 ELISA Kit (Mouse) (OKEH05270) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Apoc2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Apoc2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Apoc2 captured in the well.
ELISA Kit Range0.312-20ng/mL
ELISA Kit RecoveryMean recovery when spiking into Serum and Plasma = 102.1%
ELISA Kit ReproducibilityThree samples concentrations were measured in replicate within an assay plate and across replicate assays to assess Intra- and Mean Inter-Assay Precision.
Mean Intra-Assay Precision - <6.8%, n = 20
Mean Inter-Assay Precision - <9.4%, n = 20
ELISA Kit Component
ComponentAmount
Apoc2 Microplate96 Wells (12 x 8 Well strips)
Apoc2 Lyophilized Standard2
100X Biotinylated Apoc2 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
Stop Solution1 x 10 mL
TMB Substrate1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, plasma and other biological fluids
Sensitivity0.156 ng/mL
SpecificityNatural and recombinant Mouse Apolipoprotein C-II
Publications

CREBH normalizes dyslipidemia and halts atherosclerosis in diabetes by decreasing circulating remnant lipoproteins. J Clin Invest. 131 (2021). 34491909

Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Description
Gene SymbolApoc2
Gene Full Nameapolipoprotein C2
Alias SymbolsapoC-II;apo-CII;Apolipoprotein C2;apolipoprotein C-II.
NCBI Gene Id11813
Protein NameQ05020
Description of TargetApolipoprotein C-II
Uniprot IDhttps://www.uniprot.org/uniprot/Q05020
Protein Accession #NP_001264873.1
Nucleotide Accession #NM_001277944.1
Protein Size (# AA)97
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