APOBEC3G Antibody (OAPB00226)

Catalog No: OAPB00226 (Formerly GWB-7FF4D8)

Size:100UG

Western blot analysis of APOBEC3G expression in Caco-2 cell lysate in the (A), absence and (B) presence of blocking peptide with APOBEC3G antibody at 5 μg/mL.

Immunohistochemical staining of human spleen using APOBEC3G antibody at 1 μg/mL.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid. APOBEC3G Antibody is supplied in PBS containing 0.02% sodium azide.
• Clonality: Polyclonal
• Host: Rabbit
• Conjugation: Unconjugated
• Application: Enzyme-linked immunosorbent assay|Immunohistochemistry-Paraffin|Western blot
  Buffer: Antibody is supplied in PBS containing 0.02% sodium azide.
• Reconstitution and Storage: APOBEC3G antibody can be stored at 4C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Immunogen: APOBEC3G antibody was raised against a synthetic peptide corresponding to 15 amino acids near the amino-terminus of human APOBEC3G. The immunogen is located within the first 50 amino acids of APOBEC3G.
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Human
• Concentration: 1 mg/ml
• Blocking Peptide: For anti-APOBEC3G (OAPB00226) antibody is Catalog # OEPB02320
• Specificity: APOBEC3G antibody will also detect the APOBEC3F isoform.
• Application Info: APOBEC3G antibody can be used for detection of APOBEC3G by Western blot at 5 ug/ml. (Optimal dilution should be determined by user)
• Reference: Jarmuz A, Chester A, Bayliss J, et al. An anthropoid-specific locus of Orphan C to U RNA-editing enzymes on chromosome 22. Genomics 2002; 79:285-96.;Stremlau M, Owens CM, Perron MJ, et al. The cytoplasmic body component TRIM5alpha restricts HIV-1 infection in Old World monkeys. Nature 2004; 427:848-53.;Bieniasz PD. Intrinsic immunity: a front-line defense against viral attack. Nat Immunol. 2004; 5:1109-15.;Sheehy AM, Gaddis NC, Choi JD, et al. Isolation of a human gene that inhibits HIV-1 infection and is suppressed by the viral Vif protein. Nature 2002; 418:646-50.

Target Info

• Gene Symbol: APOBEC3G
• Gene Full Name: apolipoprotein B mRNA editing enzyme catalytic subunit 3G
• Alias Symbols: A3G;APOBEC-related cytidine deaminase;APOBEC-related protein 9;apolipoprotein B editing enzyme catalytic polypeptide-like 3G;apolipoprotein B mRNA editing enzyme cytidine deaminase;apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3G;apolipoprotein B mRNA-editing enzyme catalytic polypeptide 3G;ARCD;ARP9;ARP-9;bK150C2.7;CEM15;CEM-15;deoxycytidine deaminase;dJ494G10.1;DNA dC->dU editing enzyme;DNA dC->dU-editing enzyme APOBEC-3G;MDS019;phorbolin-like protein MDS019.
• NCBI Gene Id: 60489
• Protein Name: DNA dC->dU-editing enzyme APOBEC-3G|DNA dC->dU-editing enzyme APOBEC-3G isoform 1 [Homo sapiens]
• Description of Target: DNA deaminase (cytidine deaminase) which acts as an inhibitor of retrovirus replication and retrotransposon mobility via deaminase-dependent and -independent mechanisms. Exhibits potent antiviral activity against vif-deficient HIV-1. After the penetration of retroviral nucleocapsids into target cells of infection and the initiation of reverse transcription, it can induce the conversion of cytosine to uracil in the minus-sense single-strand viral DNA, leading to G-to-A hypermutations in the subsequent plus-strand viral DNA. The resultant detrimental levels of mutations in the proviral genome, along with a deamination-independent mechanism that works prior to the proviral integration, together exert efficient antiretroviral effects in infected target cells. Selectively targets single-stranded DNA and does not deaminate double-stranded DNA or single-or double-stranded RNA. Exhibits antiviral activity also against simian immunodeficiency viruses (SIVs), hepatitis B virus (HBV), equine infectious anemia virus (EIAV), xenotropic MuLV-related virus (XMRV) and simian foamy virus (SFV). May inhibit the mobility of LTR and non-LTR retrotransposons.
• Uniprot ID: Q9HC16
• Protein Accession #: https://www.ncbi.nlm.nih.gov/protein/NP_068594
• Nucleotide Accession #: NM_021822.3