APOBEC3D/F Antibody (OAAF04061)

Catalog No: OAAF04061 (Formerly GWB-ASE869)

Size:100 ug

Western blot analysis of extracts from COS7/MCF-7 cells, using APOBEC3D/F Antibody. The lane on the right is treated with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human placenta tissue, using APOBEC3D/F Antibody. The picture on the right is treated with the synthesized peptide.

Product Info

• Predicted Species Reactivity: Human|Monkey
• Product Format: Liquid
• Clonality: Polyclonal
• Host: Rabbit
• Application: Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin|Western blot
• Reconstitution and Storage: -20°C
• Immunogen: The antiserum was produced against synthesized peptide derived from human APOBEC3D/F.
• Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using immunogen.
• Specificity: APOBEC3D/F antibody detects endogenous levels of total APOBEC3D/F protein.
• Formulation: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Dilution: The optimal working dilution should be determined by the end user.
• Application Info: WB: 1:500~1:1000; IHC: 1:50~1:100; ELISA: 1:40000
• Reference: Dunham I., Nature 402:489-495(1999).
  Jarmuz A., Genomics 79:285-296(2002).
  Wedekind J.E., Trends Genet. 19:207-216(2003).

Target Info

• Gene Symbol: APOBEC3D|APOBEC3F
• Gene Full Name: apolipoprotein B mRNA editing enzyme catalytic subunit 3D|apolipoprotein B mRNA editing enzyme catalytic subunit 3F
• Alias Symbols: A3D;A3DE;A3F;APOBEC3DE;APOBEC3E;apolipoprotein B editing enzyme catalytic polypeptide-like 3F;apolipoprotein B mRNA editing enzyme cytidine deaminase;apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3D;apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3E pseudogene;apolipoprotein B mRNA-editing enzyme catalytic polypeptide-like 3F;ARP6;ARP8;BK150C2.4.MRNA;DNA dC->dU-editing enzyme APOBEC-3D;DNA dC->dU-editing enzyme APOBEC-3F;induced upon T-cell activation;KA6;probable DNA dC->dU-editing enzyme APOBEC-3D.
• NCBI Gene Id: 140564|200316
• Protein Name: DNA dC->dU-editing enzyme APOBEC-3D|DNA dC->dU-editing enzyme APOBEC-3F
• Description of Target: DNA deaminase (cytidine deaminase) which acts as an inhibitor of retrovirus replication and retrotransposon mobility via deaminase-dependent and -independent mechanisms. Exhibits antiviral activity against vif-deficient HIV-1. After the penetration of retroviral nucleocapsids into target cells of infection and the initiation of reverse transcription, it can induce the conversion of cytosine to uracil in the minus-sense single-strand viral DNA, leading to G-to-A hypermutations in the subsequent plus-strand viral DNA. The resultant detrimental levels of mutations in the proviral genome, along with a deamination-independent mechanism that works prior to the proviral integration, together exert efficient antiretroviral effects in infected target cells. Selectively targets single-stranded DNA and does not deaminate double-stranded DNA or single- or double-stranded RNA. Exhibits antiviral activity also against hepatitis B virus (HBV), equine infectious anemia virus (EIAV), xenotropic MuLV-related virus (XMRV) and simian foamy virus (SFV) and may inhibit the mobility of LTR and non-LTR retrotransposons. May also play a role in the epigenetic regulation of gene expression through the process of active DNA demethylation.|DNA deaminase (cytidine deaminase) which acts as an inhibitor of retrovirus replication and retrotransposon mobility via deaminase-dependent and -independent mechanisms. Exhibits antiviral activity against vif-deficient HIV-1. After the penetration of retroviral nucleocapsids into target cells of infection and the initiation of reverse transcription, it can induce the conversion of cytosine to uracil in the minus-sense single-strand viral DNA, leading to G-to-A hypermutations in the subsequent plus-strand viral DNA. The resultant detrimental levels of mutations in the proviral genome, along with a deamination-independent mechanism that works prior to the proviral integration, together exert efficient antiretroviral effects in infected target cells. Selectively targets single-stranded DNA and does not deaminate double-stranded DNA or single-or double-stranded RNA. May inhibit the mobility of LTR and non-LTR retrotransposons.
• Uniprot ID: Q8IUX4|Q96AK3
• Protein Accession #: NP_001006667.1
• Molecular Weight: 46 kDa