APOB48 High Sensitivity ELISA Kit (Human) (OKCD01355)

Catalog No: OKCD01355

Size:96WELLS

Human APOB48 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	98-105%	83-90%	87-94%	78-89%
  EDTA Plasma (n=5)	79-94%	82-101%	94-104%	85-104%
  Heparin Plasma (n=5)	79-93%	92-99%	98-105%	94-102%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to High Sensitive Apolipoprotein B48 (APOB48). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to High Sensitive Apolipoprotein B48 (APOB48). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain High Sensitive Apolipoprotein B48 (APOB48), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of High Sensitive Apolipoprotein B48 (APOB48) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 2.4-150ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	82-91	88
  EDTA Plasma (n=5)	86-101	95
  Heparin Plasma (n=5)	98-105	102

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level High Sensitive Apolipoprotein B48 (APOB48) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level High Sensitive Apolipoprotein B48 (APOB48) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-APOB48 Microplate: 96 Wells (12 x 8 Well Strips)
  • APOB48 Lyophilized Standard: 2
  • 100X Biotinylated APOB48 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: < 1.1 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Apolipoprotein B48 (APOB48). No significant cross-reactivity or interference between Apolipoprotein B48 (APOB48) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: APOB
• Gene Full Name: apolipoprotein B
• Alias Symbols: apoB-100;apoB-48;apolipoprotein B (including Ag(x) antigen);apolipoprotein B-100;apolipoprotein B48;FCHL2;FLDB;LDLCQ4.
• NCBI Gene Id: 338
• Protein Name: Apolipoprotein B-100
• Description of Target: Apolipoprotein B is a major protein constituent of chylomicrons (apo B-48), LDL (apo B-100) and VLDL (apo B-100). Apo B-100 functions as a recognition signal for the cellular binding and internalization of LDL particles by the apoB/E receptor.
• Uniprot ID: P04114