APOB High Sensitivity ELISA Kit (Human) (OKCD01363)

Catalog No: OKCD01363

Size:96WELLS

Human APOB ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	85-103%	94-103%	89-96%	80-95%
  EDTA Plasma (n=5)	98-105%	95-103%	78-93%	98-105%
  Heparin Plasma (n=5)	91-104%	95-102%	78-90%	91-103%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to High Sensitive Apolipoprotein B (APOB). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to High Sensitive Apolipoprotein B (APOB). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain High Sensitive Apolipoprotein B (APOB), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of High Sensitive Apolipoprotein B (APOB) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 1.56-100ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	78-92	81
  EDTA Plasma (n=5)	89-99	93
  Heparin Plasma (n=5)	90-97	94

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level High Sensitive Apolipoprotein B (APOB) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level High Sensitive Apolipoprotein B (APOB) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-APOB Microplate: 96 Wells (12 x 8 Well Strips)
  • APOB Lyophilized Standard: 2
  • 100X Biotinylated APOB Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Sensitivity: 0.65 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Apolipoprotein B (APOB). No significant cross-reactivity or interference between Apolipoprotein B (APOB) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: APOB
• Gene Full Name: apolipoprotein B
• Alias Symbols: apoB-100;apoB-48;apolipoprotein B (including Ag(x) antigen);apolipoprotein B-100;apolipoprotein B48;FCHL2;FLDB;LDLCQ4.
• NCBI Gene Id: 338
• Protein Name: Apolipoprotein B-100
• Description of Target: Apolipoprotein B is a major protein constituent of chylomicrons (apo B-48), LDL (apo B-100) and VLDL (apo B-100). Apo B-100 functions as a recognition signal for the cellular binding and internalization of LDL particles by the apoB/E receptor.
• Uniprot ID: P04114
• Protein Accession #: NP_000375.2
• Nucleotide Accession #: NM_000384.2