Catalog No: OKCA00135
Size:96 Wells
Price: $525.00
SKU
OKCA00135
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APOB ELISA Kit (Mouse) : 96 Wells (OKCA00135)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
COA DatasheetClick Hereto download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityMouse
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration2.5 Hours
ELISA Kit Linearity
Sample Type 1
Sample TypeDilutionAverage %Range %
Serum (n=4)1:100102%97-108%
Serum (n=4)1:200103%97-106%
Serum (n=4)1:40092%88-97%
Serum (n=4)1:80086%82-91%
ELISA Kit PrincipleAviva Systems Biology APOB ELISA Kit (Mouse) (OKCA00135) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with Mouse APOB and blocked. Standards or samples are added to the plate wells along with a horseradish peroxidase (HRP) conjugated antibody specific to APOB and incubated. APOB immobilized in the wells competes with the liquid phase APOB contained in the samples or standards for binding with the HRP-Conjugated APOB Antibody. Wells are washed removing unbound APOB, unbound HRP-Conjugated APOB Antibody or APOB/HRP-Conjugated APOB Antibody complex. HRP-Conjugated APOB Antibody bound to APOB immobilized in the well remains. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of HRP-Conjugated APOB Antibody captured in the well and inversely proportional to the amount of APOB which was contained in the sample or standard.
ELISA Kit Range0.156 ug/mL - 10 ug/mL
ELISA Kit Recovery
Sample 1
Sample TypeAverage RecoveryRange
Serum (n=5)93%90-95%
Sample 2
Sample TypeAverage RecoveryRange
EDTA Plasma (n=4)94%90-98%
ELISA Kit ReproducibilityIntra-assay: CV% < 8% (n = 20). Inter-assay: CV% < 10% (n = 20)
ELISA Kit Component
ComponentAmount
APOB Microplate96 Well (12 x 8 Well Strips)
APOB Standard (Lyophilized)2 x 10 ug
100X HRP-Conjugate1 x 60 uL
HRP-Conjugate Diluent1 x 10 mL
Sample Diluent2 x 20 mL
25X Wash Buffer1 x 20 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates
Sensitivity< 0.039 ug/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of
mouse APOB. No significant cross-reactivity or interference between mouse APOB and analogues was observed.
Assay InfoAssay Methodology: Quantitative Competitive Immunoassay
Gene SymbolAPOB
Gene Full NameApolipoprotein B
Alias Symbolsapob-, apob-48, AI315052, apob-100, Apo B-100
NCBI Gene Id238055
Protein NameApolipoprotein B-100
Description of TargetThis gene product is the main apolipoprotein of chylomicrons and low density lipoproteins. It occurs in plasma as two main isoforms, apoB-48 and apoB-100. Unlike the apoB-48 and apoB-100 structural equivalents in human, which are synthesized exclusively in the gut and liver, respectively, the mouse apoB-48 isoform is also found in mouse liver. The intestinal and the hepatic forms of apoB are encoded by a single gene from a single, very long mRNA. The two isoforms share a common N-terminal sequence. The shorter apoB-48 protein is produced after RNA editing of the apoB-100 transcript at residue 2179 (CAA->UAA), resulting in the creation of a stop codon, and early translation termination.
Uniprot IDE9Q414
Protein Accession #NP_033823
Nucleotide Accession # NM_009693
Protein Size (# AA)4505
Molecular Weight509 kDa
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