APOB Chemi-Luminescent ELISA Kit (Human) (OKCD05571)

Catalog No: OKCD05571

Size:96WELLS

APOB Chemi-Luminescent ELISA Kit (Human) (OKCD05571) Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Chemiluminescent immunoassay
• ELISA Kit Detection Method: Chemiluminescent
• ELISA Kit Duration: 2h, 40min
• ELISA Kit Principle: The microplate provided in this kit has been pre-coated with an antibody specific to Apolipoprotein B48 (APOB48). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Apolipoprotein B48 (APOB48). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Apolipoprotein B48 (APOB48) level in the sample or standard.
• ELISA Kit Range: 0.69-500pg/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Apolipoprotein B48 (APOB48) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Apolipoprotein B48 (APOB48) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • APOB Microplate: 96 Wells (12 x 8 Well strips)
  • APOB Lyophilized Standard: 2
  • 100X APOB HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • 100X Luminol Substrate: 1 x 9 mL
  • Substrate Diluent: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma and other biological fluids.
• Sensitivity: < 0.28pg/mL

Target Info

• Gene Symbol: APOB
• Gene Full Name: apolipoprotein B
• Alias Symbols: apoB-100;apoB-48;apolipoprotein B (including Ag(x) antigen);apolipoprotein B-100;apolipoprotein B48;FCHL2;FLDB;LDLCQ4.
• NCBI Gene Id: 338
• Protein Name: Apolipoprotein B-100
• Description of Target: Apolipoprotein B is a major protein constituent of chylomicrons (apo B-48), LDL (apo B-100) and VLDL (apo B-100). Apo B-100 functions as a recognition signal for the cellular binding and internalization of LDL particles by the apoB/E receptor.
• Uniprot ID: P04114
• Protein Accession #: NP_000375.2
• Nucleotide Accession #: NM_000384.2