APOB Chemi-Luminescent ELISA Kit (Human) (OKCD03303)

Catalog No: OKCD03303

Size:96WELLS

Human APOB ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Chemiluminescent immunoassay
• ELISA Kit Detection Method: Chemiluminescent
• ELISA Kit Duration: 2h, 40min
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	98-105%	97-104%	79-96%	99-105%
  EDTA Plasma (n=5)	79-102%	79-101%	80-99%	98-105%
  Heparin Plasma (n=5)	79-101%	95-103%	87-97%	85-97%

• ELISA Kit Principle: The microplate provided in this kit has been pre-coated with an antibody specific to Apolipoprotein B (APOB). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Apolipoprotein B (APOB). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Apolipoprotein B (APOB) level in the sample or standard.
• ELISA Kit Range: 6.86-5,000ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	90-99	96
  EDTA Plasma (n=5)	83-101	92
  Heparin Plasma (n=5)	93-104	98

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Apolipoprotein B (APOB) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Apolipoprotein B (APOB) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • APOB Microplate: 96 Wells (12 x 8 Well strips)
  • APOB Lyophilized Standard: 2
  • 100X APOB Biotinylated Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • 100X Luminol Substrate: 1 x 2 mL
  • Substrate Diluent: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Sensitivity: 6.86 ng/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: APOB
• Gene Full Name: apolipoprotein B
• Alias Symbols: apoB-100;apoB-48;apolipoprotein B (including Ag(x) antigen);apolipoprotein B-100;apolipoprotein B48;FCHL2;FLDB;LDLCQ4.
• NCBI Gene Id: 338
• Protein Name: Apolipoprotein B-100
• Description of Target: Apolipoprotein B is a major protein constituent of chylomicrons (apo B-48), LDL (apo B-100) and VLDL (apo B-100). Apo B-100 functions as a recognition signal for the cellular binding and internalization of LDL particles by the apoB/E receptor.
• Uniprot ID: P04114
• Protein Accession #: NP_000375
• Nucleotide Accession #: NM_000384