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Catalog No: OKCD09560
Size:96WELLS
Price: $625.00
SKU
OKCD09560
Availability: Domestic: within 2-3 weeks delivery | International: within 2-3 weeks delivery
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Datasheets/ManualsPrintable datasheet for APOA5 ELISA Kit (Mouse) (OKCD09560)
Product Info
Predicted Species ReactivityMouse|Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Wide-range Apolipoprotein A5 (APOA5). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Wide-range Apolipoprotein A5 (APOA5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Wide-range Apolipoprotein A5 (APOA5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Wide-range Apolipoprotein A5 (APOA5) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range1.56-100ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Wide-range Apolipoprotein A5 (APOA5) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Wide-range Apolipoprotein A5 (APOA5) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
APOA5 Microplate96 Wells (12 x 8 Well strips)
APOA5 Lyophilized Standard2
100X APOA5 HRP-Detector Anitbody1 x 120 ÂuL
100X Avidin-HRP Conjugate1 x 120 ÂuL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 2 mL
Stop Solution1 x 20 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity< 0.67ng/mL
Publications

CREBH normalizes dyslipidemia and halts atherosclerosis in diabetes by decreasing circulating remnant lipoproteins. J Clin Invest. 131 (2021). 34491909

Description
Gene SymbolApoa5
Gene Full Nameapolipoprotein A-V
Alias Symbols1300007O05Rik;Apoav;apolipoprotein A5;apolipoprotein A-V;R;RAP3;regeneration-associated protein 3.
NCBI Gene Id66113
Protein NameApolipoprotein A-V
Description of TargetMinor apolipoprotein mainly associated with HDL and to a lesser extent with VLDL. May also be associated with chylomicrons. Important determinant of plasma triglyceride (TG) levels by both being a potent stimulator of apo-CII lipoprotein lipase (LPL) TG hydrolysis and a inhibitor of the hepatic VLDL-TG production rate (without affecting the VLDL-apoB production rate). Activates poorly lecithin:cholesterol acyltransferase (LCAT) and does not enhance efflux of cholesterol from macrophages (By similarity).
Uniprot IDQ8C7G5
Protein Accession #NP_001335024.1
Nucleotide Accession #NM_001348095.1
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