APOA5 ELISA Kit (Mouse) (OKCD09560)

Catalog No: OKCD09560

Size:96 Wells

APOA5 ELISA Kit (Mouse) (OKCD09560) Standard Curve

Product Info

• Predicted Species Reactivity: Mouse
• Application: ELISA
• ELISA Kit Detection Method: Colorimetric, OD 450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology APOA5 ELISA Kit (Mouse) (OKCD09560) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for APOA5 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for APOA5 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample APOA5 captured in the well.
• ELISA Kit Range: 1.56-100ng/mL
• ELISA Kit Reproducibility: Intra-assay reproducibility was evaluated using 20 replicates of 3 samples representing low, middle and high level APOA5. Inter-assay reproducibility was evaluated with 3 samples representing low, middle and high level APOA5 using 8 replicates on each of 3 plates.
  Intra-Assay CV <10%
  Inter-Assay CV <12%
• ELISA Kit Component:
  • APOA5 Microplate: 96 Wells (12 x 8 Well strips)
  • APOA5 Lyophilized Standard: 2
  • 100X APOA5 HRP-Detector Anitbody: 1 x 120 ÂuL
  • 100X Avidin-HRP Conjugate: 1 x 120 ÂuL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 2 mL
  • Stop Solution: 1 x 20 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Sensitivity: < 0.67ng/mL
• Publications:

  CREBH normalizes dyslipidemia and halts atherosclerosis in diabetes by decreasing circulating remnant lipoproteins. J Clin Invest. 131 (2021). 34491909

Target Info

• Gene Symbol: APOA5
• Gene Full Name: apolipoprotein A5
• Alias Symbols: R, RAP3, Apoav, 1300007O05Rik
• NCBI Gene Id: 66113
• Protein Name: apolipoprotein A-V
• Description of Target: Minor apolipoprotein mainly associated with HDL and to a lesser extent with VLDL. May also be associated with chylomicrons. Important determinant of plasma triglyceride (TG) levels by both being a potent stimulator of apo-CII lipoprotein lipase (LPL) TG hydrolysis and a inhibitor of the hepatic VLDL-TG production rate (without affecting the VLDL-apoB production rate). Activates poorly lecithin:cholesterol acyltransferase (LCAT) and does not enhance efflux of cholesterol from macrophages.
• Uniprot ID: Q8C7G5
• Protein Accession #: NP_001335024.1
• Nucleotide Accession #: NM_001348095.1