Apoa4 High Sensitivity ELISA Kit (Mouse) (OKCD01357)

Catalog No: OKCD01357

Size:96WELLS

Mouse Apoa4 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	94-102%	90-97%	78-103%	78-96%
  EDTA Plasma (n=5)	94-105%	80-102%	99-105%	79-104%
  Heparin Plasma (n=5)	90-103%	80-99%	81-92%	90-97%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to High Sensitive Apolipoprotein A4 (APOA4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to High Sensitive Apolipoprotein A4 (APOA4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain High Sensitive Apolipoprotein A4 (APOA4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of High Sensitive Apolipoprotein A4 (APOA4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 7.8-500pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	88-104	97
  EDTA Plasma (n=5)	88-99	94
  Heparin Plasma (n=5)	78-92	87

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level High Sensitive Apolipoprotein A4 (APOA4) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level High Sensitive Apolipoprotein A4 (APOA4) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-Apoa4 Microplate: 96 Wells (12 x 8 Well Strips)
  • Apoa4 Lyophilized Standard: 2
  • 100X Biotinylated Apoa4 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: 2.7 pg/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Apolipoprotein A4 (APOA4). No significant cross-reactivity or interference between Apolipoprotein A4 (APOA4) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Apoa4
• Gene Full Name: apolipoprotein A-IV
• Alias Symbols: Ap;Apoa-4;apoA-IV;apo-AIV;apolipoprotein A4;apolipoprotein A-IV.
• NCBI Gene Id: 11808
• Protein Name: Apolipoprotein A-IV
• Description of Target: May have a role in chylomicrons and VLDL secretion and catabolism. Required for efficient activation of lipoprotein lipase by ApoC-II; potent activator of LCAT. Apoa-IV is a major component of HDL and chylomicrons.
• Uniprot ID: P06728
• Protein Accession #: NP_031494.2
• Nucleotide Accession #: NM_007468.2