Catalog No: OKCD00678 (Formerly GWB-KBBYU3)
Size:96WELLS
Price: $650.00
SKU
OKCD00678
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Datasheets/ManualsPrintable datasheet for APOA4 ELISA Kit (Mouse) (OKCD00678)
Product Info
Predicted Species ReactivityMouse|Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
serum(n=5)86-99%82-96%92-101%78-98%
EDTA plasma(n=5)78-102%93-102%79-101%97-105%
heparin plasma(n=5)87-102%93-105%91-105%90-102%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Apolipoprotein A4 (APOA4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Apolipoprotein A4 (APOA4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Apolipoprotein A4 (APOA4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Apolipoprotein A4 (APOA4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.39-25ng/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
serum(n=5)84-10295
EDTA plasma(n=5)80-9184
heparin plasma(n=5)89-10398
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Apolipoprotein A4 (APOA4) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Apolipoprotein A4 (APOA4) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-Apoa4 Microplate96 Wells (12 x 8 Well Strips)
Apoa4 Lyophilized Standard2 x 100 ng
100X Biotinylated Apoa4 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity< 0.69 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Apolipoprotein A4 (APOA4). No significant cross-reactivity or interference between Apolipoprotein A4 (APOA4) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolApoa4
Gene Full Nameapolipoprotein A-IV
Alias SymbolsAp;Apoa-4;apoA-IV;apo-AIV;apolipoprotein A4;apolipoprotein A-IV.
NCBI Gene Id11808
Protein NameApolipoprotein A-IV
Description of TargetMay have a role in chylomicrons and VLDL secretion and catabolism. Required for efficient activation of lipoprotein lipase by ApoC-II; potent activator of LCAT. Apoa-IV is a major component of HDL and chylomicrons.
Uniprot IDP06728
Protein Accession #NP_031494
Nucleotide Accession #NM_007468.2
Protein Size (# AA)395
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