APOA2 ELISA Kit (Mouse) (OKCA02159)

Catalog No: OKCA02159

Size:96 Wells

Bovine HMGB1 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Mouse
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3.5 Hours
• ELISA Kit Principle: Aviva Systems Biology Apoa2 ELISA Kit (Mouse) (OKCA02159) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for APOA2 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for APOA2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is read by absorbance at 450 nm and is quantitatively proportional to the amount of sample APOA2 captured in well.
• ELISA Kit Range: 3.9 ng/mL-250 ng/mL
• ELISA Kit Reproducibility: Mean Intra-Assay Variation <10% CV (n=20)
  Mean Inter-Assay Variation <12% CV (n=8)
• ELISA Kit Component:
  • Anti-APOA2 Microplate: 96 Wells (12 x 8 Well strips)
  • APOA2 Lyophilized Standard: 2 x 250 ng
  • 100X Biotinylated APOA2 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 50 mL
  • Detector Antibody Diluent: 1 x 15 mL
  • Conjugate Diluent: 1 x 15 mL
  • 25X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 10 mL
  • TMB Substrate: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates
• Sensitivity: 0.98 ng/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: APOA2
• Alias Symbols: A, Al, Ap, Hdl-, Alp-2, Hdl-1, ApoAII, Apoa-2, Apo-AII, ApoA-II
• NCBI Gene Id: 11807
• Protein Name: Apolipoprotein A-II
• Description of Target: May stabilize HDL (high density lipoprotein) structure by its association with lipids, and affect the HDL metabolism.
• Uniprot ID: P09813
• Protein Accession #: NP_001292478
• Nucleotide Accession #: NM_001305549