Catalog No: OKCD02375
Size:96WELLS
Price: $725.00
SKU
OKCD02375
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Datasheets/ManualsPrintable datasheet for APOA1 ELISA Kit (Pig) (OKCD02375)
Product Info
Predicted Species ReactivityPorcine|Sus scrofa
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:21:41:81:16
Serum (n=5)84-96%89-101%89-97%86-101%
EDTA Plasma (n=5)88-102%82-103%81-95%80-102%
Heparin Plasma (n=5)91-105%91-98%83-97%88-95%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Apolipoprotein A1 (APOA1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Apolipoprotein A1 (APOA1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Apolipoprotein A1 (APOA1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Apolipoprotein A1 (APOA1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range28.12-1800ng/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average(%)
Serum (n=5)83-9387
EDTA Plasma (n=5)82-9891
Heparin Plasma(n=5)94-10298
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Apolipoprotein A1 (APOA1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Apolipoprotein A1 (APOA1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-APOA1 Microplate96 Wells (12 x 8 Well strips)
APOA1 Lyophilized Standard2
100X Biotinylated APOA1 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
Stop Solution1 x 6 mL
TMB Substrate1 x 9 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernatants and other biological fluids
Sensitivity< 1.43 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Apolipoprotein A1 (APOA1). No significant cross-reactivity or interference between Apolipoprotein A1 (APOA1) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Protocol Information
Gene SymbolAPOA1
Gene Full Nameapolipoprotein A1
Alias SymbolsapoA-I;apo-AI;Apolipoprotein A1;apolipoprotein A-I.
NCBI Gene Id397691
Protein NameApolipoprotein A-I
Description of TargetParticipates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT). As part of the SPAP complex, activates spermatozoa motility.
Uniprot IDP18648
Protein Accession #NP_999563.1
Nucleotide Accession #NM_214398.1
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