Catalog No: OKEH02643
Price: $425.00
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APOA1 ELISA Kit (Human) : 96 Wells (OKEH02643)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
COA DatasheetPrintable COA datasheet for OKEH02643
Product Info
Predicted Species ReactivityHomo sapiens, Human
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology APOA1 ELISA Kit (Human) (OKEH02643) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for APOA1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for APOA1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample APOA1 captured in well.
ELISA Kit Range7.8-500ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 91%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 4.8% (n = 20)
Mean Inter-assay CV%: < 7.6% (n = 20)
ELISA Kit Component
Anti-APOA1 Microplate96 Wells (12 x 8 Well strips)
APOA1 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated APOA1 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity3.4 ng/mL
SpecificityNatural and recombinant Human Apolipoprotein A-I
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolAPOA1
Gene Full Nameapolipoprotein A1
Alias Symbolsapo(a);apo-AI;Apolipoprotein A1;apolipoprotein A-I;epididymis secretory sperm binding protein;HPALP2.
NCBI Gene Id335
Protein NameP02647
Description of TargetApolipoprotein A-I
Uniprot ID
Protein Accession #NP_000030.1
Nucleotide Accession #NM_000039.2
Protein Size (# AA)267
Molecular Weight31 kDa
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