APOA1 Chemi-Luminescent ELISA Kit (Human) (OKCD03300)

Catalog No: OKCD03300

Size:96WELLS

Human APOA1 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Chemiluminescent immunoassay
• ELISA Kit Detection Method: Chemiluminescent
• ELISA Kit Duration: 2h, 40min
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	83-97%	90-104%	97-105%	99-105%
  EDTA Plasma (n=5)	95-102%	80-93%	78-96%	98-105%
  Heparin Plasma (n=5)	79-88%	81-103%	98-105%	89-97%

• ELISA Kit Principle: The microplate provided in this kit has been pre-coated with an antibody specific to Apolipoprotein A1 (APOA1). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Apolipoprotein A1 (APOA1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Apolipoprotein A1 (APOA1) level in the sample or standard.
• ELISA Kit Range: 1.37-1,000ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	78-96	93
  EDTA Plasma (n=5)	81-97	87
  Heparin Plasma (n=5)	95-102	98

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Apolipoprotein A1 (APOA1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Apolipoprotein A1 (APOA1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • APOA1 Microplate: 96 Wells (12 x 8 Well strips)
  • APOA1 Lyophilized Standard: 2
  • 100X APOA1 Biotinylated Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • 100X Luminol Substrate: 1 x 2 mL
  • Substrate Diluent: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Sensitivity: 1.37- 1,000 ng/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: APOA1
• Gene Full Name: apolipoprotein A1
• Alias Symbols: apo(a);apo-AI;Apolipoprotein A1;apolipoprotein A-I;epididymis secretory sperm binding protein;HPALP2.
• NCBI Gene Id: 335
• Protein Name: Apolipoprotein A-I
• Description of Target: Participates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT). As part of the SPAP complex, activates spermatozoa motility.
• Uniprot ID: P02647
• Protein Accession #: NP_000030
• Nucleotide Accession #: NM_000039