APLNR ELISA Kit (Rat) (OKEH03572)

Catalog No: OKEH03572

Size:96W

Rat APLNR ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Rat|Rattus norvegicus
• Application: ELISA-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology APLNR ELISA Kit (Rat) (OKEH03572) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Aplnr has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Aplnr is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Aplnr captured in well.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Component:
  • Aplnr Microplate: 96 Wells (12 x 8 Well strips)
  • Aplnr Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • 100X Biotinylated Aplnr Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 10 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 0.175 ng/mL
• Specificity: Natural and recombinant Rat Apelin receptor
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Aplnr
• Gene Full Name: apelin receptor
• Alias Symbols: Agtrl1, angiotensin receptor-like 1, apelin receptor, Apj, B78, G-protein coupled receptor APJ, GPCR34.
• NCBI Gene Id: 83518
• Protein Name: Apelin receptor
• Description of Target: Receptor for apelin receptor early endogenous ligand (APELA) and apelin (APLN) hormones coupled to G proteins that inhibit adenylate cyclase activity (PubMed:11359874). Plays a key role in early development such as gastrulation, blood vessels formation and heart morphogenesis by acting as a receptor for APELA hormone. May promote angioblast migration toward the embryonic midline, i.e. the position of the future vessel formation, during vasculogenesis. Promotes sinus venosus (SV)-derived endothelial cells migration into the developing heart to promote coronary blood vessel development. Plays also a role in various processes in adults such as regulation of blood vessel formation, blood pressure, heart contractility and heart failure (By similarity).
• Uniprot ID: Q9JHG3
• Protein Accession #: NP_112639.1
• Nucleotide Accession #: NM_031349.2
• Protein Size (# AA): 377
• Molecular Weight: 42 kDa