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Catalog No: OKEH01327
Size:96T
Price: $525.00
SKU
OKEH01327
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHuman
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology APLN36 ELISA Kit (Human) (OKEH01327) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for APLN36 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for APLN36 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample APLN36 captured in well.
ELISA Kit Range78-5000pg/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 86%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 4.9% (n = 20)
Mean Inter-assay CV%: < 6.2% (n = 20)
ELISA Kit Component
ComponentAmount
APLN36 Microplate96 Wells (12 x 8 Well strips)
APLN36 Lyophilized Standard2
100X Biotinylated APLN36 Detector Antibody1 x 120 ÂuL
100X Avidin-HRP Conjugate1 x 120 ÂuL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity23 pg/mL
SpecificityNatural and recombinant Human Apelin-36
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolAPLN36
Alias SymbolsAPEL, XNPEP2
NCBI Gene Id8862
Protein NameApelin
Description of TargetApelin gene encodes a pre-proprotein of 77 amino acids, with a signal peptide in the N-terminal region. After translocation into the endoplasmic reticulum and cleavage of the signal peptide, the proprotein of 55 amino acids may generate several active fragments: a 36 amino acid peptide corresponding to the sequence 42-77 (apelin 36), a 17 amino acid peptide corresponding to the sequence 61-77 (apelin 17) and a 13 amino acid peptide corresponding to the sequence 65-77 (apelin 13).
Uniprot IDQ9ULZ1
Protein Accession #NP_059109
Nucleotide Accession #NM_017413
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