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Datasheets/Manuals | Printable datasheet for API5 Antibody (OAAF02826) |
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Predicted Species Reactivity | Human|Mouse |
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Product Format | Liquid |
Clonality | Polyclonal |
Host | Rabbit |
Application | Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin|Western blot |
Reconstitution and Storage | -20°C |
Immunogen | The antiserum was produced against synthesized peptide derived from human API-5. |
Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Specificity | API-5 antibody detects endogenous levels of total API-5 protein. |
Formulation | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Dilution | The optimal working dilution should be determined by the end user. |
Application Info | WB: 1:500~1:3000; IHC: 1:50~1:100; ELISA: 1:20000 |
Reference | Tewari M., Cancer Res. 57:4063-4069(1997). Van den Berghe L., Mol. Endocrinol. 14:1709-1724(2000). Olsen J.V., Cell 127:635-648(2006). |
Gene Symbol | API5 |
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Gene Full Name | apoptosis inhibitor 5 |
Alias Symbols | AAC11;AAC-11;antiapoptosis clone 11 protein;apoptosis inhibitor 5;cell migration-inducing gene 8 protein;Fibroblast growth factor 2-interacting factor;fibroblast growth factor 2-interacting factor 2;FIF;migration-inducing protein MIG8;Protein XAGL. |
NCBI Gene Id | 8539 |
Protein Name | Apoptosis inhibitor 5 |
Description of Target | Antiapoptotic factor that may have a role in protein assembly. Negatively regulates ACIN1. By binding to ACIN1, it suppresses ACIN1 cleavage from CASP3 and ACIN1-mediated DNA fragmentation. Also known to efficiently suppress E2F1-induced apoptosis. Its depletion enhances the cytotoxic action of the chemotherapeutic drugs. |
Uniprot ID | Q9BZZ5 |
Protein Accession # | NP_001136402.1 |
Molecular Weight | 57 kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "API5 Antibody (OAAF02826)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human|Mouse".
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How long will it take to receive "API5 Antibody (OAAF02826)"?
This item is available "Domestic: within 1-2 week delivery | International: 1-2 weeks".
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What buffer format is "API5 Antibody (OAAF02826)" provided in?
This item is provided in "Liquid".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "API5 Antibody (OAAF02826)"?
This target may also be called "AAC11;AAC-11;antiapoptosis clone 11 protein;apoptosis inhibitor 5;cell migration-inducing gene 8 protein;Fibroblast growth factor 2-interacting factor;fibroblast growth factor 2-interacting factor 2;FIF;migration-inducing protein MIG8;Protein XAGL." in publications.
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What is the shipping cost for "API5 Antibody (OAAF02826)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "API5 Antibody (OAAF02826)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "API5 Antibody (OAAF02826)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "57 kDa".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "API5 Antibody (OAAF02826)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "API5"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "API5"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "API5"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "API5"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "API5"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "API5"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.