APG4B Antibody - C-terminal region (ARP34434_P050)

Catalog No: ARP34434_P050

WB Suggested Anti-APG4B Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:12500
Positive Control: Raji cell lysateATG4B is strongly supported by BioGPS gene expression data to be expressed in Human Raji cells

Human kidney

Rabbit Anti-ATG4B Antibody
Catalog Number: ARP34434
Paraffin Embedded Tissue: Human alveolar cell
Cellular Data: Epithelial cells of renal tubule
Antibody Concentration: 4.0-8.0 ug/ml
Magnification: 400X

Product Info

• Tested Species Reactivity: Human
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Clonality: Polyclonal
• Host: Rabbit
• Application: IHC, WB
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human APG4B
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Cow: 93%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 85%
• Peptide Sequence: Synthetic peptide located within the following region: LGGALPMFELVELQPSHLACPDVLNLSLDSSDVERLERFFDSEDEDFEIL
• Concentration: 0.5 mg/ml
• Blocking Peptide: For anti-ATG4B (ARP34434_P050) antibody is Catalog # AAP34434 (Previous Catalog # AAPY00336)
• Sample Type Confirmation:

  ATG4B is strongly supported by BioGPS gene expression data to be expressed in Raji

• Reference: Tanida,I., et al., (2004) J. Biol. Chem. 279 (35), 36268-36276

Target Info

• Gene Symbol: ATG4B
• Gene Full Name: ATG4 autophagy related 4 homolog B (S. cerevisiae)
• Alias Symbols: APG4B, AUTL1
• NCBI Gene Id: 23192
• Protein Name: Cysteine protease ATG4B
• Description of Target: Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. APG4B encodes a member of the autophagin protein family and is also designated as a member of the C-54 family of cysteine proteases.
• Uniprot ID: Q9Y4P1
• Protein Accession #: NP_037457
• Nucleotide Accession #: NM_013325
• Protein Size (# AA): 393
• Molecular Weight: 44kDa
• Protein Interactions: SUMO2; MAP1LC3B; MAP1LC3A; BAG3; ATG8; YWHAB; GABARAPL1; SQSTM1; UBC; GABARAPL2; ATG10; ATG3; AMBRA1; GABARAP; CAMKK2; ATG12; ULK1; STK3; KRT4; ANXA1; FBXW11; LINC00341;