Catalog No: OABB00448 (Formerly GWB-BBP371)
Size:100UG
Price: $432.00
SKU
OABB00448
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Datasheets/ManualsPrintable datasheet for APEX1 Polyclonal Antibody (OABB00448)
Product Info
Tested Species ReactivityHuman, Mouse, Rat
Predicted Species ReactivityHamster|Human|Mouse|Rat
Product FormatLyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
ClonalityPolyclonal
ClonePolyclonal
IsotypeRabbit IgG
ApplicationImmunohistochemistry|Western blot
Additional InformationNotes: Tested Species: In-house tested species with positive results.
Predicted Species: Species predicted to be fit for the product based on sequence similarities.
By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.
Other applications have not been tested.
Optimal dilutions should be determined by end users.
::Background: APEX1, also called apurinic endonuclease(APE), is a DNA repair enzyme having apurinic/apyrimidinic(AP) endonuclease, 3-prime,5-prime-exonuclease, DNA 3-prime repair diesterase, and DNA 3-prime-phosphatase activities. The human APEX1 gene consists of 5 exons spanning 2.64 kb and exists as a single copy in the haploid genome. Using in situ hybridization, the APEX1 gene is mapped to 14q11.2-q12. The predicted APEX1 protein, which contained probable nuclear transport signals, was identified as a member of a family of DNA repair enzymes found in lower organisms. The abundance of the large form of APEX1 was increased in leiomyoma extracts relative to myometrial tissue extracts, and the large form was dominant in cell lines derived from leiomyosarcomas. The exonuclease activity of nuclear APEX1 can remove the anti-HIV nucleoside analogs AZT and D4T from the 3-prime terminus of a nick more efficiently than can cytosolic exonucleases.
::Background: APEX1, also called apurinic endonuclease (APE), is a DNA repair enzyme having apurinic/apyrimidinic (AP) endonuclease, 3-prime,5-prime-exonuclease, DNA 3-prime repair diesterase, and DNA 3-prime-phosphatase activities. The human APEX1 gene consists of 5 exons spanning 2.64 kb and exists as a single copy in the haploid genome. Using in situ hybridization, the APEX1 gene is mapped to 14q11.2-q12. The predicted APEX1 protein, which contained probable nuclear transport signals, was identified as a member of a family of DNA repair enzymes found in lower organisms. The abundance of the large form of APEX1 was increased in leiomyoma extracts relative to myometrial tissue extracts, and the large form was dominant in cell lines derived from leiomyosarcomas.The exonuclease activity of nuclear APEX1 can remove the anti-HIV nucleoside analogs AZT and D4T from the 3-prime terminus of a nick more efficiently than can cytosolic exonucleases.
Reconstitution and Storage2°C to 8°C|-20°C
ImmunogenPolypeptide
PurificationAffinity Purified
Predicted Homology Based on Immunogen SequenceHuman; Mouse; Rat
Concentration500 ug/ml
SpecificityNo cross reactivity with other proteins.
Application InfoWestern blot: 0.1-0.5 ug/ml: Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section): 0.5-1 ug/ml: Human, Rat: Mouse: By Heat
Reference1. Akiyama, K., Seki, S., Oshida, T., Yoshida, M. C.Structure, promoter analysis and chromosomal
assignment of the human APEX gene.Biochim. Biophys. Acta 1219: 15-25, 1994.
2. Chou, K.-M., Cheng, Y.-C.An exonucleolytic activity of human apurinic/apyrimidinic endonuclease
on 3-prime mispaired DNA.Nature 415: 655-659, 2002.
3. Robson, C. N., Hochhauser, D., Craig, R., Rack, K., Buckle, V. J., Hickson, I. D.Structure of the
human DNA repair gene HAP1 and its localisation to chromosome 14q11.2-12.Nucleic Acids Res.
20: 4417-4421, 1992.
Storage BufferEach vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
DescriptionRabbit IgG polyclonal antibody for DNA-(apurinic or apyrimidinic site) lyase(APEX1) detection. Tested with WB, IHC-P in Human;Mouse;Rat.
Gene SymbolAPEX1
Gene Full Nameapurinic/apyrimidinic endodeoxyribonuclease 1
Alias SymbolsAP endonuclease class I;AP lyase;APE;APE1;APEN;APEX;APEX nuclease;APEX nuclease (multifunctional DNA repair enzyme) 1;apurinic/apyrimidinic (abasic) endonuclease;apurinic-apyrimidinic endonuclease 1;APX;deoxyribonuclease (apurinic or apyrimidinic);DNA-(apurinic or apyrimidinic site) endonuclease;DNA-(apurinic or apyrimidinic site) lyase;HAP1;protein REF-1;redox factor-1;REF1;REF-1.
NCBI Gene Id328
Protein NameDNA-(apurinic or apyrimidinic site) lyase
Description of TargetMultifunctional protein that plays a central role in the cellular response to oxidative stress. The two major activities of APEX1 in DNA repair and redox regulation of transcriptional factors. Functions as a apurinic/apyrimidinic (AP) endodeoxyribonuclease in the DNA base excision repair (BER) pathway of DNA lesions induced by oxidative and alkylating agents. Initiates repair of AP sites in DNA by catalyzing hydrolytic incision of the phosphodiester backbone immediately adjacent to the damage, generating a single-strand break with 5'-deoxyribose phosphate and 3'-hydroxyl ends. Does also incise at AP sites in the DNA strand of DNA/RNA hybrids, single-stranded DNA regions of R-loop structures, and single-stranded RNA molecules. Has a 3'-5' exoribonuclease activity on mismatched deoxyribonucleotides at the 3' termini of nicked or gapped DNA molecules during short-patch BER. Possesses a DNA 3' phosphodiesterase activity capable of removing lesions (such as phosphoglycolate) blocking the 3' side of DNA strand breaks. May also play a role in the epigenetic regulation of gene expression by participating in DNA demethylation. Acts as a loading factor for POLB onto non-incised AP sites in DNA and stimulates the 5'-terminal deoxyribose 5'-phosphate (dRp) excision activity of POLB. Plays a role in the protection from granzymes-mediated cellular repair leading to cell death. Also involved in the DNA cleavage step of class switch recombination (CSR). On the other hand, APEX1 also exerts reversible nuclear redox activity to regulate DNA binding affinity and transcriptional activity of transcriptional factors by controlling the redox status of their DNA-binding domain, such as the FOS/JUN AP-1 complex after exposure to IR. Involved in calcium-dependent down-regulation of parathyroid hormone (PTH) expression by binding to negative calcium response elements (nCaREs). Together with HNRNPL or the dimer XRCC5/XRCC6, associates with nCaRE, acting as an activator of transcriptional repression. Stimulates the YBX1-mediated MDR1 promoter activity, when acetylated at Lys-6 and Lys-7, leading to drug resistance. Acts also as an endoribonuclease involved in the control of single-stranded RNA metabolism. Plays a role in regulating MYC mRNA turnover by preferentially cleaving in between UA and CA dinucleotides of the MYC coding region determinant (CRD). In association with NMD1, plays a role in the rRNA quality control process during cell cycle progression. Associates, together with YBX1, on the MDR1 promoter. Together with NPM1, associates with rRNA. Binds DNA and RNA.
Uniprot IDP27695
Molecular Weight35555 MW
  1. What is the species homology for "APEX1 Polyclonal Antibody (OABB00448)"?

    The tested species reactivity for this item is "Human, Mouse, Rat". This antibody is predicted to have homology to "Hamster|Human|Mouse|Rat".

  2. How long will it take to receive "APEX1 Polyclonal Antibody (OABB00448)"?

    This item is available "Domestic: within 1-2 week delivery | International: within 1-2 week delivery".

  3. What buffer format is "APEX1 Polyclonal Antibody (OABB00448)" provided in?

    This item is provided in "Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.".
    Additional format options may be available. For more information please contact info@avivasysbio.com.

  4. What are other names for "APEX1 Polyclonal Antibody (OABB00448)"?

    This target may also be called "AP endonuclease class I;AP lyase;APE;APE1;APEN;APEX;APEX nuclease;APEX nuclease (multifunctional DNA repair enzyme) 1;apurinic/apyrimidinic (abasic) endonuclease;apurinic-apyrimidinic endonuclease 1;APX;deoxyribonuclease (apurinic or apyrimidinic);DNA-(apurinic or apyrimidinic site) endonuclease;DNA-(apurinic or apyrimidinic site) lyase;HAP1;protein REF-1;redox factor-1;REF1;REF-1." in publications.

  5. What is the shipping cost for "APEX1 Polyclonal Antibody (OABB00448)"?

    The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.

  6. What is the guarantee for "APEX1 Polyclonal Antibody (OABB00448)"?

    All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.

  7. Can I get bulk pricing for "APEX1 Polyclonal Antibody (OABB00448)"?

    You can get bulk pricing for this item by going here.

  8. What is the molecular weight of the protein?

    The molecular weight reported by Uniprot for this item is "35555 MW".
    Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics.

  9. What protocols are available for "APEX1 Polyclonal Antibody (OABB00448)"?

    We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.

  10. What are positive controls for "APEX1"?

    We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.

  11. What are negative controls for "APEX1"?

    We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.

  12. What other proteins interact with "APEX1"?

    This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.

  13. What biological processes are associated with "APEX1"?

    This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.

  14. What cellular components are associated with "APEX1"?

    This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.

  15. What protein functions are associated with "APEX1"?

    This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.

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