APEH ELISA Kit (Mouse) (OKEH05511)

Catalog No: OKEH05511

Size:96T

Mouse APEH ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Mouse, Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~3 Hours
• ELISA Kit Principle: Aviva Systems Biology Apeh ELISA Kit (Mouse) (OKEH05511) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Apeh has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Apeh is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Apeh captured in the well.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into Serum and Plasma = 93.6%
• ELISA Kit Reproducibility: Three samples concentrations were measured in replicate within an assay plate and across replicate assays to assess Intra- and Mean Inter-Assay Precision.
  Mean Intra-Assay Precision - <6.8%, n = 20
  Mean Inter-Assay Precision - <9.3%, n = 20
• ELISA Kit Component:
  • APEH Microplate: 96 Wells (12 x 8 Well strips)
  • APEH Lyophilized Standard: 2
  • 100X Biotinylated APEH Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • Stop Solution: 1 x 10 mL
  • TMB Substrate: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: 0.162 ng/mL
• Specificity: Natural and recombinant Mouse Acylamino-acid-releasing enzyme
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Apeh
• Gene Full Name: acylpeptide hydrolase
• Alias Symbols: AARE;acylamino-acid-releasing enzyme;acylaminoacyl-peptidase;acyl-peptide hydrolase;APH;N-acylaminoacyl peptide hydrolase.
• NCBI Gene Id: 235606
• Protein Name: Q8R146
• Description of Target: Acylamino-acid-releasing enzyme
• Uniprot ID: https://www.uniprot.org/uniprot/Q8R146
• Protein Accession #: NP_666338.2
• Nucleotide Accession #: NM_146226.2
• Protein Size (# AA): 732